![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0013.jpg "Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRP |
| Predicted Species | Rat (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PBK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Publication using NBP1-84342 | Applications | Species |
|---|---|---|
| Mulder J, Bjorling E, Jonasson K et al. Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics. Mol Cell Proteomics 2009-07-01 [PMID: 19351664] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PBK Antibody (NBP1-84342)Find related products by research area.
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| Gene Symbol | PBK |
![Western Blot: PBK Antibody [NBP1-84342] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP1-84342-img0011.jpg "Western Blot: PBK Antibody [NBP1-84342] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP1-84342] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0012.jpg "Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP1-84342] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human testis.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0017.jpg "Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human testis.")
![Western Blot: PBK Antibody [NBP1-84342] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP1-84342-img0010.jpg "Western Blot: PBK Antibody [NBP1-84342] - Analysis in human cell line A-431.")
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse brain shows positivity in rostral migratory stream.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0006.jpg "Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse brain shows positivity in rostral migratory stream.")
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse medulla shows positivity in a subset of cells.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0007.jpg "Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse medulla shows positivity in a subset of cells.")
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse olfactory bulb shows immunoreactivity in a subset of neurons.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0008.jpg "Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse olfactory bulb shows immunoreactivity in a subset of neurons.")
![Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse subventricular zone shows immunoreactivity in some cells in the rostral migratory stream.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84342-img0009.jpg "Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse subventricular zone shows immunoreactivity in some cells in the rostral migratory stream.")
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0014.jpg "Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon.")
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human kidney.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0015.jpg "Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human kidney.")
![Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human liver.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunohistochemistry-Paraffin-NBP1-84342-img0016.jpg "Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human liver.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![Immunocytochemistry Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Immunocytochemistry_6472.jpg)
![Simple Western Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Simple_Western_18959.jpg)
![Western Blot Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Western_Blot_18960.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Western Blot Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/Raf-1_MAB4540_Western_Blot_9426.jpg)
![Immunohistochemistry Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/Raf-1_MAB4540_Immunohistochemistry_9891.jpg)
![Simple Western Raf-1 Antibody (563002) [Unconjugated]](https://images.novusbio.com/images2/mab4540_human-mouse-rat-raf-1-mab-clone-563002-simple-western-158202212919.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg)
![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")