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PBEF/Visfatin/NAMPT Antibody

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Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6384-SP
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PBEF/Visfatin/NAMPT Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21derived recombinant rat PBEF/Visfatin
Gln25-His491
Accession # Q80Z29
Specificity
Detects rat PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse PBEF and recombinant human PBEF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NAMPT
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase/Nampt) is a 52‑54 kDa member of the NAPRTase family of molecules. It is a dimer that is found both intracellularly and extracellularly, where it participates in NAD synthesis and induces proinflammatory cytokine synthesis, respectively. PBEF has also been reported to show insulin-like effects on adipocytes by binding to the insulin receptor. Cells known to express PBEF include visceral adipocytes, neutrophils, and capillary endothelium. Rat PBEF is 491 amino acids in length. It contains no signal sequence but is secreted. Potential phosphorylation sites exist at Tyr34, Tyr188 and Ser398. Full‑length (aa 1‑491) rat PBEF shares 99% and 95% aa identity with mouse and human PBEF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PBEF/Visfatin/NAMPT An
AF6384
PBEF/Visfatin/NAMPT Antibody
Species: Rt
Applications: WB

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Bioinformatics

Gene Symbol NAMPT
Uniprot