![Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940840.jpg "Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
| Reactivity | Hu, Mu, Rt, DrSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1279 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding phospho Thr410 of human pan PKC zeta. The exact sequence is proprietary. |
| Modification | p Thr410 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PRKCA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for pan PKC Antibody (NBP3-25605)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRKCA |
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024941594.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249342742.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249422634.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249415946.jpg "Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940812.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249383155.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")