![Western Blot: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Western-Blot-NBP2-73226-img0001.jpg "Western Blot: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI4H5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human PADI4(NP_036519) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PADI4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 73.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
Secondary Antibodies |
Isotype Controls |
Research Areas for PADI4 Antibody (NBP2-73226)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PADI4 |
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73226-img0004.jpg "Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0012.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.")
![Flow Cytometry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73226-img0003.jpg "Flow Cytometry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0005.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0006.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0007.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0008.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0009.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0010.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0011.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73226-img0013.jpg "Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.")
![Flow Cytometry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73226-img0002.jpg "Flow Cytometry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Western_Blot_5296.jpg)
![Simple Western Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Simple_Western_16762.jpg)
![Immunocytochemistry Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Immunocytochemistry__Immunofluorescence_17421.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
