![Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Western-Blot-NBP2-02082-img0025.jpg "Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4H5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human PADI4(NP_036519) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PADI4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 73.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-02082 | Applications | Species |
|---|---|---|
| Santalla M�ndez R, Rodgers Furones A, Classens R et al. Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells Cell Mol Life Sci 2023-09-27 [PMID: 37755527] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PADI4 Antibody (NBP2-02082)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-02082-img0016.jpg "Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0027.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.")
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0014.jpg "Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0017.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0018.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0019.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0020.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0021.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0022.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0023.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0024.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.")
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0015.jpg "Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Western_Blot_5296.jpg)
![Simple Western Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Simple_Western_16762.jpg)
![Immunocytochemistry Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Immunocytochemistry__Immunofluorescence_17421.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
