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PAD3 Antibody

Images

 
Orthogonal Strategies: Western Blot: PAD3 Antibody [NBP1-92240] - Human cell lines RT-4 and PC-3 using Anti-PADI3 antibody. Corresponding PADI3 RNA-seq data are presented for the same cell lines. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: PAD3 Antibody [NBP1-92240] - Staining of human cell line A549 shows localization to nucleoplasma, cytosol and vesicles. Antibody staining is shown in green.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

PAD3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FRMLLASPGACFKLFQEKQKCGHGRALLFQGVVDDEQVKTISINQVLSNKDLINYNKFVQSCIDWNREV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PADI3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PAD3 Protein (NBP1-92240PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PAD3 Antibody

  • EC 3.5.3.15
  • MGC126307
  • PAD3
  • PDI3MGC126308
  • peptidyl arginine deiminase, type III
  • Peptidylarginine deiminase III
  • Protein-arginine deiminase type III
  • protein-arginine deiminase type-3

Background

PADI3 / PAD3 catalyzes the deimination of arginine residues of proteins. It belongs to the protein arginine deiminase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PAD3 Antibody (NBP1-92240) (0)

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Bioinformatics

Gene Symbol PADI3
Entrez
Uniprot