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PSMD14 Antibody

Images

 
Western Blot: PSMD14 Antibody [NBP1-80956] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PSMD14 Antibody [NBP1-80956] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: PSMD14 Antibody [NBP1-80956] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Western Blot: PSMD14 Antibody [NBP1-80956] - Analysis in human cell line HepG2.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PSMD14 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PSMD14 Protein (NBP1-80956PEP)
Publications
Read Publications using
NBP1-80956 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24556687)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMD14 Antibody

  • EC 3.1.2.15
  • EC 3.4.19.-
  • pad1
  • POH126S proteasome non-ATPase regulatory subunit 14,26S proteasome regulatory subunit RPN11
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
  • Rpn11
  • RPN11,26S proteasome-associated PAD1 homolog 1

Background

PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMD14 Antibody (NBP1-80956)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMD14 Antibody (NBP1-80956) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMD14
Entrez
Uniprot