![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Western blotting showing TP53INP1 protein levels in NIH-H460 cells treated LNA-663. TUBULIN was used as protein loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-017-0080-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NBP1-76638-img0004.jpg "Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Western blotting showing TP53INP1 protein levels in NIH-H460 cells treated LNA-663. TUBULIN was used as protein loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-017-0080-x) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised with a synthetic peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1. The immunogen is located within the first 50 amino acids of p53DINP1. Amino Acid Squence: MFQYLNKMFVGEVS |
| Specificity | At least two isoforms of p53DINP1 are known to exist; this antibody will detect both isoforms. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TP53INP1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
jennifer guadagno |
WB | Mouse | 10/06/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for p53 DINP1 Antibody (NBP1-76638)Find related products by research area.
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![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 u/mL.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NBP1-76638-img0001.jpg "Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 u/mL.")
![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - miR-663 controls PIN-1-mediated apoptosis & p53 mitochondrial functiona Immunoblot showing BTG2 downmodulation 72 h after siRNA transfection. TUBULIN was used as protein loading control. Normalized BTG2 levels are expressed as fold decrease relative to cntrl treated cells. b Western blotting showing BTG2 modulation in NIH-H460 after the indicated treatments. Double transfection of anti-BTG2 siRNA together with LNA-663 restores BTG2 levels of control cells, counteracting LNA-mediated induction. PARP-1 cleavage is reduced to 60%. TUBULIN was used as protein loading control. Numbers indicate BTG2 levels fold change relative to cntrl treated cells, & cleaved PARP-1 levels fold decrease relative to LNA-663/cntrl siRNA-treated cells. The values were normalized to TUBULIN signal, as measured by densitometric analyses. c Subcellular BTG2 & PIN-1 localization in NIH-H460 cells treated with control LNA or LNA-663 was assessed by immunofluorescence staining. d Mitochondrial membrane potential of NIH-H460 cells upon miR-663 downmodulation was measured by JC-1 staining. An increase of the ratio between green & red signals is indicative of membrane depolarization typical of apoptotic cells. e Western blotting showing PIN-1 modulation in NIH-H460 subcellular fractions 24 h after LNA-663 treatment. Cytoplasmic accumulation & nuclear decrease of PIN-1 are expressed as fold changes relative to cntrl-treated cells. The histogram shows the ratio between cytoplasmic & nuclear signal. f Immunoblot of p53 in NIH-H460 subcellular fractions. Hsp90 & Bcl-2 were used as cytoplasmic & mitochondrial markers, respectively. Mitochondrial p53 modulation is expressed as fold increase relative to cntrl treated cells & normalized to Bcl-2 signal Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29352138), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-31020241616377.jpg "Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - miR-663 controls PIN-1-mediated apoptosis & p53 mitochondrial functiona Immunoblot showing BTG2 downmodulation 72 h after siRNA transfection. TUBULIN was used as protein loading control. Normalized BTG2 levels are expressed as fold decrease relative to cntrl treated cells. b Western blotting showing BTG2 modulation in NIH-H460 after the indicated treatments. Double transfection of anti-BTG2 siRNA together with LNA-663 restores BTG2 levels of control cells, counteracting LNA-mediated induction. PARP-1 cleavage is reduced to 60%. TUBULIN was used as protein loading control. Numbers indicate BTG2 levels fold change relative to cntrl treated cells, & cleaved PARP-1 levels fold decrease relative to LNA-663/cntrl siRNA-treated cells. The values were normalized to TUBULIN signal, as measured by densitometric analyses. c Subcellular BTG2 & PIN-1 localization in NIH-H460 cells treated with control LNA or LNA-663 was assessed by immunofluorescence staining. d Mitochondrial membrane potential of NIH-H460 cells upon miR-663 downmodulation was measured by JC-1 staining. An increase of the ratio between green & red signals is indicative of membrane depolarization typical of apoptotic cells. e Western blotting showing PIN-1 modulation in NIH-H460 subcellular fractions 24 h after LNA-663 treatment. Cytoplasmic accumulation & nuclear decrease of PIN-1 are expressed as fold changes relative to cntrl-treated cells. The histogram shows the ratio between cytoplasmic & nuclear signal. f Immunoblot of p53 in NIH-H460 subcellular fractions. Hsp90 & Bcl-2 were used as cytoplasmic & mitochondrial markers, respectively. Mitochondrial p53 modulation is expressed as fold increase relative to cntrl treated cells & normalized to Bcl-2 signal Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29352138), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunofluorescence of p53 DINP1 in human liver tissue with p53 DINP1 antibody at 5 ug/ml. Green: p53 DINP1 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202419353887.gif "Immunocytochemistry/ Immunofluorescence: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunofluorescence of p53 DINP1 in human liver tissue with p53 DINP1 antibody at 5 ug/ml. Green: p53 DINP1 antibody Red: Phylloidin staining Blue: DAPI staining")
![Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunohistochemical staining of mouse liver using p53 DINP1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202420352418.jpg "Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunohistochemical staining of mouse liver using p53 DINP1 antibody at 2 ug/mL.")
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