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Osteopontin/OPN Antibody

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Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human placenta and liver tissues. Corresponding SPP1 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human placenta and liver tissues. Corresponding SPP1 RNA-seq data are presented for the same tissues.
Western Blot: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human kidney shows strong positivity in cytoplasm and secretion in tubules.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human liver shows low positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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Osteopontin/OPN Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDAT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
Control Peptide
Osteopontin/OPN Protein (NBP1-89952PEP)
Publications
Read Publication using NBP1-89952.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Osteopontin/OPN Antibody

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Osteopontin/OPN
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NBP1-89952
Osteopontin/OPN Antibody
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Applications: WB, ICC/IF, IHC

Publications for Osteopontin/OPN Antibody (NBP1-89952)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPP1