Osteopontin/OPN Antibodies
We offer Osteopontin/OPN Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Neutralization, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each Osteopontin/OPN Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Osteopontin/OPN Antibodies can be used in a variety of model species: Canine, Human, Mouse, Porcine, Rabbit, Rat. Use the list below to choose the Osteopontin/OPN Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Osteopontin/OPN polyclonal antibodies and browse our Osteopontin/OPN monoclonal antibody catalog.
Alternate Names for Osteopontin/OPN Antibodies
anti-Osteopontin/OPN antibody, anti-SPP1 antibody, anti-BNSP antibody, anti-Bone sialoprotein 1 antibody, anti-MGC110940 antibody, anti-Nephropontin antibody, anti-osteopontin antibody, anti-secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1) antibody, anti-secreted phosphoprotein-1 (osteopontin, bone sialoprotein) antibody, anti-SPP-1 antibody, anti-SPP1/CALPHA1 fusion antibody, anti-Urinary stone protein antibody, anti-uropontin antibody
26 Results for "osteopontin-opn" in Primary Antibodies
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Western-Blot-NB110-89062-img0007.jpg "Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89062-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Immunohistological results at 12 weeks for (A) Group I, (B) Group II (C) Group III.The pictures are arranged by staining technique (columns) and by the investigated treatment (rows). Areas that stained positive for osteopontin are indicated by red arrowheads. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/3513) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0012.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteogenic differentiation in 3D. Immunostaining for osteopontin (OPN) and nuclear DAPI in 3D cultures after 5 weeks. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34948393/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0013.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0009.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks. The pictures are arranged by staining technique (columns) and the investigated treatment (rows). Areas that stained positive for osteocalcin and osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/3513) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0011.jpg)
![Simple Western: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Simple-Western-NB110-89062-img0010.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-3102024153560.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-31020241534321.jpg)
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-310202415394515.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB600-1043-img0009.jpg "Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.")
![Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043] - Osteopontin/OPN/OPN antibody was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin/OPN is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin/OPN is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-NB600-1043-img0010.jpg)
![Osteopontin/OPN Antibody [NB600-1043]](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-N-A-NB600-1043-img0008.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human placenta and liver tissues. Corresponding SPP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human placenta and liver tissues. Corresponding SPP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Osteopontin/OPN Antibody [NBP1-89952] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-89952-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89952-img0012.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human kidney shows strong positivity in cytoplasm and secretion in tubules.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0018.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0007.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0016.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0017.jpg)
![Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Western-Blot-NBP2-45954-img0009.jpg "Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.")
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human prostate tissue using SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0001.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0002.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0003.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0004.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0005.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0006.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0007.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0002.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-59190-img0003.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0004.jpg)