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OGG1 Antibody (2B4) - Azide and BSA Free

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Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton ...read more
Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select ...read more
Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select ...read more
Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.9 mg/ml

Order Details

OGG1 Antibody (2B4) - Azide and BSA Free Summary

Immunogen
Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300) [Uniprot: O15527]
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
OGG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.9 mg/ml
Purity
Protein A or G purified

Alternate Names for OGG1 Antibody (2B4) - Azide and BSA Free

  • 8-hydroxyguanine DNA glycosylase
  • 8-oxoguanine DNA glycosylase
  • AP lyase
  • DNA-apurinic or apyrimidinic site lyase
  • HOGG1
  • MMH
  • MUTMOGH1HMMH
  • N-glycosylase/DNA lyase
  • OGG1

Background

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on OGG1.

You down with Ogg? Yeah, you know me
8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,...  Read full blog post.

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Bioinformatics

Gene Symbol OGG1