![Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Analysis of extracts of various cell lines, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Western-Blot-NBP3-03454-img0004.jpg "Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Analysis of extracts of various cell lines, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | POLB |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DNA Polymerase beta Antibody (NBP3-03454)Find related products by research area.
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PARP Antibody Assays aid both Apoptosis and Cancer Research The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play... Read full blog post. |
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| Gene Symbol | POLB |
![Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP3-03454] - Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (NBP3-03454). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03454-img0003.jpg "Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP3-03454] - Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (NBP3-03454). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Mouse kidney using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-03454-img0002.jpg "Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Mouse kidney using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Human stomach using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-03454-img0001.jpg "Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Human stomach using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).")
![Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Knockout-Validated-NBP3-03454-img0005.jpg "Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")