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Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated]

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Western blot shows lysates of TK-6 human lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Nesfatin-1/ Nucleobindin-2 between 10 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of TK-6 human lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) ...read more
Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5949-SP
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Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nesfatin‑1/Nucleobindin‑2
Pro26-Leu106
Accession # P80303
Specificity
Detects human Nesfatin‑1/Nucleobindin‑2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NUCB2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF5949.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated]

  • DNA-binding protein NEFA
  • NEFA
  • NEFAGastric cancer antigen Zg4
  • Nesfatin1
  • Nesfatin-1
  • NUCB2
  • nucleobindin 2
  • Nucleobindin-2
  • nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1-24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF5949
Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated]
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Applications: WB, IHC, ICC/IF

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Bioinformatics

Gene Symbol NUCB2
Uniprot