![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
| Reactivity | Hu, Mu, Pm-OrSpecies Glossary |
| Applications | WB, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by Nbs1 Antibody maps to a region between residue 704 and 754 of human Nibrin using the numbering given in entry NP_002476.2 (GeneID 4683). |
| Predicted Species | Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NBN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 23852118 and 24036913). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Theoretical MW | 85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nbs1 Antibody (NB100-60654)Find related products by research area.
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The MRE11 Complex and DNA Damage Response The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra... Read full blog post. |
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NBS1: The DNA Repair Trigger NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch... Read full blog post. |
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NBS1: DNA Repair Trigger NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch... Read full blog post. |
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![Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.")
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