We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?

Name: Nbs1 Antibody (1D7)
Brand: Novus Biologicals
SKU: NB100-221
Rating: 5 (3 reviews)

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I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Simple Western, ICC/IF, IHC, IP, KD
Clone	1D7
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	The complete coding region of the human p95/Nbs1 expressed in E. coli.
Localization	Nuclear
Specificity	Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. Nbs1 Antibody (1D7) recognizes the 95kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	NBN
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	No Preservative
Purity	Protein G purified

Publications using NB100-221	Applications	Species
Volcic M, Sparrer KMJ, Koepke L et al. Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1 Nat Microbiol 2020-07-20 [PMID: 32690953] (WB, Human)	WB	Human
Fagan-Solis KD, Simpson DA, Kumar RJ et al. A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability Cell Rep 2020-02-04 [PMID: 32023457] (KD, WB, Mouse)	KD, WB	Mouse
Li CG, Mahon C, Sweeney NM et al. PPARg Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis Cell Rep 2019-01-29 [PMID: 30699358] (WB, Human)	WB	Human
Choi SH, Hong HK, Cho YB et al. Identification of Sestrin3 Involved in the In vitro Resistance of Colorectal Cancer Cells to Irinotecan. PLoS ONE. 2015-05-15 [PMID: 25973791] (WB, Human)	WB	Human
Moudry P, Lukas C, Macurek L et al. Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1. Cell Death Differ 2012-05-01 [PMID: 22075984] (ICC/IF, WB, IF/IHC, Human)	ICC/IF, WB, IF/IHC	Human
E X, Pickering MT, Debatis M et al. An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus. PLoS Pathog 2011-05-01 [PMID: 21589897] (IF/IHC, WB, ICC/IF, Human)	IF/IHC, WB, ICC/IF	Human
Demogines A, East AM, Lee JH et al. Ancient and recent adaptive evolution of primate non-homologous end joining genes. PLoS Genet 2010-10-01 [PMID: 20975951] (ICC/IF, WB, IF/IHC, Human)	ICC/IF, WB, IF/IHC	Human
Inoue S-ichi, Yoshinari K, Sugawara M, Yamazoe Y. Activated sterol regulatory element-binding protein-2 suppresses hepatocyte nuclear factor-4-mediated Cyp3a11 expression in mouse liver. Mol. Pharmacol. 79(1):148-156. 2011-01-01 [PMID: 20926756]
Cha H, Lowe JM, Li H et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res 2010-05-01 [PMID: 20460517] (IF/IHC, WB, ICC/IF, Human)	IF/IHC, WB, ICC/IF	Human
Mandriota SJ, Buser R, Lesne L et al. Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells. J Biol Chem 2010-04-01 [PMID: 20177072] (IF/IHC, ICC/IF, WB, Human)	IF/IHC, ICC/IF, WB	Human
Lee JH, Goodarzi AA, Jeggo PA et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J 2010-02-01 [PMID: 20010693] (WB, ICC/IF, IF/IHC, Human)	WB, ICC/IF, IF/IHC	Human
Lins S, Kim R, Kruger L et al. Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome. Gene 2009-11-01 [PMID: 19635536] (IF/IHC, WB, ICC/IF, Human)	IF/IHC, WB, ICC/IF	Human
Collaco RF, Bevington JM, Bhrigu V et al. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology 2009-09-01 [PMID: 19628243] (ICC/IF, WB, IF/IHC, Human)	ICC/IF, WB, IF/IHC	Human
Abuzeid W M, Jiang X et al. Molecular disruption of RAD50 sensitizes human tumor cells to cisplatin-based chemotherapy. J Clin Invest 2009-01-07 [PMID: 19487811] (WB, Human)	WB	Human
Bartkova J, Tommiska J, Oplustilova L et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol 2008-12-01 [PMID: 19383352] (WB, ICC/IF, IF/IHC, Human)	WB, ICC/IF, IF/IHC	Human
Zheng L, Kanagaraj R, Mihaljevic B et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 2009-05-01 [PMID: 19270065] (WB, ICC/IF, IF/IHC, Human)	WB, ICC/IF, IF/IHC	Human
Spycher C, Miller ES, Townsend K et al. Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin. J Cell Biol 2008-04-01 [PMID: 18411308] (IF/IHC, ICC/IF, WB, Human)	IF/IHC, ICC/IF, WB	Human
den Dulk B, van Eijk P, de Ruijter M et al. The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4. DNA Repair (Amst) 2008-06-01 [PMID: 18387345] (IF/IHC, WB, ICC/IF, Human)	IF/IHC, WB, ICC/IF	Human
Takemura H, Rao VA, Sordet O et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem 2006-10-01 [PMID: 16905549] (IF/IHC, WB, ICC/IF, Human)	IF/IHC, WB, ICC/IF	Human
Kruger L, Demuth I, Neitzel H et al. Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein. Carcinogenesis 2007-01-01 [PMID: 16840438] (WB, IF/IHC, ICC/IF, Human)	WB, IF/IHC, ICC/IF	Human
Cerosaletti K, Concannon P. Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm. J Biol Chem 2004-09-01 [PMID: 15234984] (ICC/IF, WB, IF/IHC, Human)	ICC/IF, WB, IF/IHC	Human
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Nbs1 Antibody (1D7)

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	Verified Customer 12/22/2016
Application:	ICC
Species:	Human

	Verified Customer 12/22/2016
Application:	IP
Species:	Human

	De-identified L 04/30/2014
Application:	WB
Species:	Human

Gene Symbol	NBN
Entrez	4683(Human)
Uniprot	O60934()