![Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunohistochemistry-Paraffin-NBP2-30954-img0008.jpg "Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: PAPEAPKEPAFDPKSVKIDFTADQIE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYL4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MYL4 Antibody (NBP2-30954)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: MYL4 Antibody [NBP2-30954] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30954-img0006.jpg "Immunocytochemistry/Immunofluorescence: MYL4 Antibody [NBP2-30954] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunohistochemistry-Paraffin-NBP2-30954-img0012.jpg "Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunohistochemistry-Paraffin-NBP2-30954-img0009.jpg "Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human cerebral cortex shows no positivity in neurons as expected.")
![Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunohistochemistry-Paraffin-NBP2-30954-img0010.jpg "Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/MYL4-Antibody-Immunohistochemistry-Paraffin-NBP2-30954-img0011.jpg "Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")
![Simple Western: MYL4 Antibody [NBP2-30954] - Simple Western lane view shows a specific band for MYL4 in 0.2 mg/ml of HEL (left) and h. Heart (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MYL4-Antibody-Simple-Western-NBP2-30954-img0003.jpg "Simple Western: MYL4 Antibody [NBP2-30954] - Simple Western lane view shows a specific band for MYL4 in 0.2 mg/ml of HEL (left) and h. Heart (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: MYL4 Antibody [NBP2-30954] - Electropherogram image of the corresponding Simple Western lane view. MYL4 antibody was used at 1:50 dilution on HEL and h. Heart lysate(s) respectively.](http://images.novusbio.com/fullsize/MYL4-Antibody-Simple-Western-NBP2-30954-img0004.jpg "Simple Western: MYL4 Antibody [NBP2-30954] - Electropherogram image of the corresponding Simple Western lane view. MYL4 antibody was used at 1:50 dilution on HEL and h. Heart lysate(s) respectively.")
![Western Blot: MYL4 Antibody [NBP2-30954] -Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/antibody/nbp2-30954_rabbit-polyclonal-myl4-antibody-western-blot-1272024112446..jpg "Western Blot: MYL4 Antibody [NBP2-30954] -Analysis in human cell line HEL.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")