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HepaCAM Antibody (419305) [Unconjugated]

 (15 Publications)
 (1 Review)
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Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) ...read more
HepaCAM associates with connexin 43.(A) U373 MG cells were stably transfected with pcDNA3.1 vector, wild-type hepaCAM, hepaCAM-R92Q and hepaCAM-R92W. Immunofluorescent staining was performed with antibodies against the ...read more
HepaCAM regulates connexin 43 stability.(A) Evaluation of connexin 43 mRNA expression. Total RNA was analyzed by RT-PCR. GAPDH and no template controls (NTC) were included as housekeeping gene and negative controls, ...read more
HepaCAM regulates connexin 43 stability.(A) Evaluation of connexin 43 mRNA expression. Total RNA was analyzed by RT-PCR. GAPDH and no template controls (NTC) were included as housekeeping gene and negative controls, ...read more
HepaCAM enhances connexin 43 expression.(A) Treatment of hepaCAM-expressing U373 MG cells with antibodies against the hepaCAM extracellular domain prevents the association of hepaCAM with connexin 43 at cell-cell ...read more
HepaCAM associates with connexin 43.(A) U373 MG cells were stably transfected with pcDNA3.1 vector, wild-type hepaCAM, hepaCAM-R92Q and hepaCAM-R92W. Immunofluorescent staining was performed with antibodies against the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
419305
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4108-SP
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HepaCAM Antibody (419305) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human HepaCAM
Val34-Tyr242
Accession # Q14CZ8
Specificity
Detects human HepaCAM in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HEPACAM
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB4108 in the following application:
Publications
Read Publications using
MAB4108 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HepaCAM Antibody (419305) [Unconjugated]

  • cancer susceptibility
  • FLJ25530
  • glial cell adhesion molecule
  • GlialCAM
  • HepaCAM
  • hepatic and glial cell adhesion molecule
  • hepatocyte cell adhesion molecule
  • Protein hepaCAM

Background

HepaCAM (hepatocyte cell adhesion molecule) is a 50-75 kDa, type I transmembrane glycoprotein that belongs to the Ig-superfamily. It forms homodimers on the cell surface and promotes cell spreading and motility. Human HepaCAM is 382 aa in length. It contains a 206 aa extracellular domain (ECD) (aa 35‑240) and a 152 aa cytoplasmic region. The ECD has two C2 Ig-like domains. There is one potential truncated isoform that shows an alternate start site at Met200. Over aa 34‑242, human HepaCAM shares 99% and 98% aa sequence identity with mouse and dog HepaCAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HepaCAM Antibody
(419305) [Unconjugate ...
MAB4108
HepaCAM Antibody (419305) [Unconjugated]
Species: Hu
Applications: WB

Publications for HepaCAM Antibody (MAB4108)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Transgenic Mouse, Xenograft.

We have publications tested in 8 applications: Bioassay, Cell Culture, Flow Cytometry, IHC, Immunohistochemistry, Immunopanning, Immunoprecipitation, Western Blot.


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Bioassay
(1)
Cell Culture
(1)
Flow Cytometry
(1)
IHC
(3)
Immunohistochemistry
(1)
Immunopanning
(3)
Immunoprecipitation
(1)
Western Blot
(1)
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Human
(6)
Mouse
(3)
Transgenic Mouse
(1)
Xenograft
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB4108 Applications Species
Wang, M;Zhang, L;Novak, SW;Yu, J;Gallina, IS;Xu, LL;Lim, CK;Fernandes, S;Shokhirev, MN;Williams, AE;Saxena, MD;Coorapati, S;Parylak, SL;Quintero, C;Molina, E;Andrade, LR;Manor, U;Gage, FH; Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain Nature biotechnology 2024-02-28 [PMID: 38418648] (Immunohistochemistry, Human, Xenograft) Immunohistochemistry Human, Xenograft
Jin, S;Chen, X;Tian, Y;Jarvis, R;Promes, V;Yang, Y; Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation Nature communications 2023-08-24 [PMID: 37620511] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Voss, AJ;Lanjewar, SN;Sampson, MM;King, A;Hill, EJ;Sing, A;Sojka, C;Bhatia, TN;Spangle, JM;Sloan, SA; Identification of ligand-receptor pairs that drive human astrocyte development Nature neuroscience 2023-07-17 [PMID: 37460808] (Immunopanning, Human) Immunopanning Human
MC Krawczyk, L Pan, AJ Zhang, Y Zhang Lymphocyte deficiency alters the transcriptomes of oligodendrocytes, but not astrocytes or microglia PLoS ONE, 2023-02-24;18(2):e0279736. 2023-02-24 [PMID: 36827449] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
L Pan, A Trimarco, AJ Zhang, K Fujimori, Y Urade, LO Sun, C Taveggia, Y Zhang Oligodendrocyte-lineage cell exocytosis and L-type prostaglandin D synthase promote oligodendrocyte development and myelination Elife, 2023-02-13;12(0):. 2/13/2023 [PMID: 36779701]
J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation Cell Reports, 2023-01-05;42(1):111942. 2023-01-05 [PMID: 36640327] (IHC, Human) IHC Human
A Nolle, I van Dijken, CM Waelti, D Calini, J Bryois, E Lezan, S Golling, A Augustin, L Foo, JJM Hoozemans Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning Frontiers in Cellular Neuroscience, 2021-12-13;15(0):772011. 2021-12-13 [PMID: 34966261] (Immunopanning, Human) Immunopanning Human
KA Guttenplan, MK Weigel, P Prakash, PR Wijewardha, P Hasel, U Rufen-Blan, AE Münch, JA Blum, J Fine, MC Neal, KD Bruce, AD Gitler, G Chopra, SA Liddelow, BA Barres Neurotoxic reactive astrocytes induce cell death via saturated lipids Nature, 2021-10-06;0(0):. 2021-10-06 [PMID: 34616039] (Bioassay, Mouse) Bioassay Mouse
Zizheng Li, Ibrar Siddique, Inesa Hadrović, Abbna Kirupakaran, Jiwen Li, Ye Zhang et al. Lysine-selective molecular tweezers are cell penetrant and concentrate in lysosomes Communications Biology 9/14/2021 [PMID: 34521989]
Jiwen Li, Lin Pan, William G. Pembroke, Jessica E. Rexach, Marlesa I. Godoy, Michael C. Condro et al. Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes Nature Communications 6/25/2021 [PMID: 34172753]
Show All 15 Publications.

Review for HepaCAM Antibody (MAB4108) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using MAB4108:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Pig
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry HepaCAM MAB4108
Enlarge 		4
reviewed by:
Verified Customer
 ICC Pig 06/07/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedpig SC cells
SpeciesPig
LotN/A

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Immunocytochemistry HepaCAM MAB4108
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 Verified Customer
06/07/2017
Application: ICC
Species: Pig
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Bioinformatics

Gene Symbol HEPACAM
Uniprot