MuRF1/TRIM63 Antibody

RING domain in proteins is a zinc finger motif which serves as a protein-protein interaction interface. The muscle specific RING finger protein (MURF-1) that binds specifically to tintin repeats A168/A169 adjecent to the tintin kinase domain of the giant myofibrillar protein (1). There are 3 genes encoding three different type of muscle specific RING finger proteins (MURF1-3). Muscles adapts to decrease loads and activity by undergoing atrophy. Though in rat atrophy model majority of the genes are up-regulated, but interestingly, two sets of genes (MURF1 and MAFbx) both encode for E3 ubiquitin ligases. Knockout mice for MURF1 and MAFbx (Atrogin) exhibit resistance to muscle atrophy in quadriceps muscle after lesion of its innervation (2). Both MURF1 and MAFbx are potential therapeutic targets for muscle atrophy. E3 ubiquitin ligase. regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. E3 ubiquitin ligase can be classified into two types based on their mechanism of transferring ubiquitin to the substrate. One type, which comprises most E3s, contains a Ring motif (or the related U-box motif) and facilitates transfer of the activated ubiquitin directly from the E2 to the substrate. The second type contains a HECT domain, which forms a thioester intermediate with ubiquitin and then transfers the ubiquitin to the substrate (3). Eukaryotic cells contain a large number of different E3s (probably hundreds), and each E3 is believed to recognize only a limited number of proteins as substrates. In most cases the E3 recognizes a specific structure or amino acid sequence in the substrate (often termed the "degron") (3). For example, the "N-end rule" E3, Ubr-1, recognizes particular types of amino acids at the N terminus of the substrates (4), whereas the E3 SCFCdc4 complex recognizes a specific phosphorylated amino acid sequence in its substrate, Sic1 (4). MURF- selective antibodies were generated against peptides form unique regions on MURF1 protein corresponding to amino acid 274-293 near the C-terminal end of protein. This region was not only antigenic but also lies on the surface of the protein in native or in partially denatured MURF1 confirmation

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array NBP2-11061

• MuRF1/TRIM63 Antibodies
• MuRF1/TRIM63 Proteins