Mre11 Antibody (12D7)

Mre11 (double-strand break repair protein MRE11A) is an important component of MRN/X complex (Mre11, Rad50, and Nbs1/Xrs2), playing a central role in double-strand break (DSB) repair, DNA recombination, telomere integrity maintenance, meiotic cell division, and viral infection. Mre11 and Rad50 comprise the catalytic component of the MRN complex with Rad50 involved in ATP hydrolysis and Mre11 functioning as a nuclease having both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activity (1). The MRN complex is also involved in DNA damage signaling via activation of ATM kinase and in telomeres, where it may modulate t-loop formation (2). In addition, Mre11 is a component of the BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN) and interacts with DCLRE1C/Artemis as well as DCLRE1B/Apollo (3).

The MRN assembles as a hetero-hexamer consisting of 2 RAD50 subunits, 2 MRE11 nucleases, and 2 NBS1 subunits, specific to eukaryotes. Mre11 is highly conserved with the N-terminal region of the human protein sharing approximately 50% amino acid sequence identity with the yeast ortholog. Human Mre11 has 3 known isoforms with the predominant isoform having a theoretical molecular weight of 80.6kDa. This nuclear localized protein has high expression in proliferating tissues. Defects in Mre11 can lead to genomic instability, telomere shortening, aberrant meiosis, hypersensitivity to DNA damage, and ataxia telangiectasia-like disorder (ATLD). MRN expression has been associated with the prognosis of cancer and MRN mutants have been linked to cancer susceptibility in ovarian cancer and glioma (4, 5).

References

1. Paull TT. (2018) 20 Years of Mre11 Biology: No End in Sight. Mol Cell. 71(3):419-427. PMID: 30057197

2. Syed A, Tainer JA. (2018) The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair. Annu Rev Biochem. 87:263-294. PMID: 29709199

3. Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14(8):927-39. PMID: 10783165

4. Kim JH, Grosbart M, Anand R, Wyman C, Cejka P, Petrini JHJ. (2017) The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression. Cell Rep. 18(2):496-507. PMID: 28076792

5. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 18(1):169. PMID: 31767017

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, Immunocytochemistry/ Immunofluorescence, WB.

Showing Publications 1 - 10 of 25. Show All 25 Publications. Collapse Publications.

Publications using NB100-473	Applications	Species
Schuhwerk H, Kleemann J, Gupta P et al. The EMT transcription factor ZEB1 governs a fitness-promoting but vulnerable DNA replication stress response Cell reports 2022-12-13 [PMID: 36516781] (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Xiao H, Li F, Mladenov E et al. Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ Cells 2022-07-02 [PMID: 35805183] (WB, Human)	WB	Human
M Kusi, M Zand, LL Lin, M Chen, A Lopez, CL Lin, CM Wang, ND Lucio, NB Kirma, J Ruan, TH Huang, K Mitsuya 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity Cell Reports, 2022-01-11;38(2):110220. 2022-01-11 [PMID: 35021081]
Chansel-Da Cruz M, Hohl M, Ceppi I et al. A Disease-Causing Single Amino Acid Deletion in the Coiled-Coil Domain of RAD50 Impairs MRE11 Complex Functions in Yeast and Humans Cell reports 2020-12-29 [PMID: 33378670] (WB, Human)	WB	Human
de Vries MR, Parma L, Peters EAB et al. Blockade of VEGF receptor 2 inhibits intraplaque hemorrhage by normalization of plaque neovessels J. Intern. Med. 2018-08-13 [PMID: 30102798] (IF/IHC, Mouse)	IF/IHC	Mouse
Soares D, Battistella A, Rocca C et al. Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906 Biochem J 2011-07-01 [PMID: 21470188] (WB, Human)	WB	Human
Yamasaki S, Miura Y et al. Development of a method for effective amplification of human adenovirus 40. Arch Virol 2010-01-07 [PMID: 20490608] (WB, Human)	WB	Human
Zheng L, Kanagaraj R, Mihaljevic B et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 2009-05-01 [PMID: 19270065]
Helmink BA, Bredemeyer AL, Lee BS et al. MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks. J Exp Med 2009-03-01 [PMID: 19221393]
Ottini, L et al. MRE11 expression is impaired in gastric cancer with microsatellite instability. Carinogenesis 25: 2337-2343. 2004-01-01 [PMID: 15319296]
Giannini G, Rinaldi C, Ristori E et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene 2004-04-01 [PMID: 15048091]
Uziel T, Lerenthal Y, Moyal L et al. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J 2003-10-01 [PMID: 14532133]
Xu X, Stern DF. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J 2003-10-01 [PMID: 14519663]
Lee, H et al. Distinct functions of nijmegen breakage syndromw in ataxia telangiectasia mutated-dependent responses to DNA damage. Mol Cancer Res. 1(9):674-81. 2003-07-01 [PMID: 12861053]
Celeste, A et al. Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nature Cell Biol 5:675-679. 2003-07-01 [PMID: 12792649]
Stewart GS, Wang B, Bignell CR et al. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 2003-02-01 [PMID: 12607005]
Lukas, C et al. Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nature Cell Biol 5:255-260. 2003-01-01 [PMID: 12598907]
Xu, X et al. Is a chromatin-associeated protein involved in DNA damage signaling pathways. J Biol Chem 278:8795-8803. 2003-01-01 [PMID: 12499369]
Nakanishi K, Taniguchi T, Ranganathan V et al. Interaction of FANCD2 and NBS1 in the DNA damage response. Nat Cell Biol 2002-12-01 [PMID: 12447395]
Stracker TH, Carson CT, Weitzman MD. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature 2002-07-01 [PMID: 12124628]
Limoli, CL et al. UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-str breaks, gamma-H2AX formation, Mre11 relocalization. PNAS 99:233-238. 2002-01-01 [PMID: 11756691]
Harfst E, Cooper S, Neubauer S et al. Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome. Mol Immunol 2000-10-01 [PMID: 11282395] (WB)	WB
Lombard, DB et al. Nijmegen breakage syndrome disease protein Mre11 at PML nuclear bodies meiotic telomeres. Cancer Res 60:2331-2334. 2000-01-01 [PMID: 10811102]
Gatei, M et al. ATM-dependent phosphorylation of nibrin in response to radiation exposure. Nature Genetics 25:115-119. 2000-01-01 [PMID: 10802669]
Wang Y, Cortez D, Yazdi P et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev 2000-04-01 [PMID: 10783165]
Show All 25 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	ICC	Human	06/28/2022	View Summary Application Immunocytochemistry Sample Tested lymphoblastoid cells Species Human Lot 12D7 Comments Comments Immunocytochemistry dilution 1:200.
	5 reviewed by: Verified Customer	ICC	Mouse	12/04/2021	View Summary Application Immunocytochemistry Sample Tested fibroblasts Species Mouse Lot 12D7
Enlarge	5 reviewed by: Verified Customer	ICC	Human	12/22/2016	View Summary Application Immunocytochemistry Sample Tested primary human cells Species Human
Enlarge	5 reviewed by: De-identified L	WB	Human	04/30/2014	View Summary Application Western Blot Sample Tested HEK293 whole cell lysates Species Human

Array NB100-473

• Mre11 Antibodies
• Mre11 Lysate
• Mre11 Proteins