![Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of MMP13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Western-Blot-NBP2-66954-img0009.jpg "Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of MMP13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: MCF-7 cell lysate")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JF0893 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human MMP-13 aa 390-430. (SwissProt: P45452 Human) |
| Localization | Extracellular matrix, Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MMP13 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MMP-13 Antibody (NBP2-66954)Find related products by research area.
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| Gene Symbol | MMP13 |
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0008.jpg "Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0006.jpg "Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Flow-Cytometry-NBP2-66954-img0001.jpg "Flow Cytometry: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0002.jpg "Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0003.jpg "Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0004.jpg "Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0005.jpg "Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.")
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![N/A MMP-2 [HRP]](https://images.novusbio.com/images2/MMP2_MMP200_ELISA_474.jpg)
![N/A MMP-3 [HRP]](https://images.novusbio.com/images2/DATA_MMP3_DMP300_ELISA_767.jpg)
![N/A MMP-3 [HRP]](https://images.novusbio.com/images2/MMP-3_DMP300_ELISA_145.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/MMP-9_DMP900_ELISA_148.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/DATA_MMP9_DMP900_ELISA_770.jpg)
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![Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]](https://images.novusbio.com/images2/MMP-1_MAB901_Immunohistochemistry_7049.jpg)
![Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]](https://images.novusbio.com/images2/MMP-1_MAB901_Immunohistochemistry_9677.jpg)
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![N/A TIMP-1 [HRP]](https://images.novusbio.com/images2/TIMP-1_DTM100_ELISA_194.jpg)
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![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Flow Cytometry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]](https://images.novusbio.com/images2/MMP-14_MAB9181_Flow_Cytometry_11258.jpg)
![Immunohistochemistry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]](https://images.novusbio.com/images2/mab9181_human-mmp-14-mt1-mmp-mab-clone-128527-immunohistochemistry-239202215152.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A TIMP-2 [HRP]](https://images.novusbio.com/images2/DATA_TIMP2_DTM200_ELISA_848.jpg)
![N/A TIMP-2 [HRP]](https://images.novusbio.com/images2/DATA_TIMP2_DTM200_ELISA_849.jpg)
![N/A TIMP-2 [HRP]](https://images.novusbio.com/images2/TIMP2_DTM200_ELISA_2722.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")