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MMP-12 Antibody

 (7 Publications)
 (2 Reviews)
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Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer ...read more
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MMP-12 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary.
Localization
Secreted , extracellular space , extracellular matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-31225 in the following applications:
Publications
Read Publications using
NBP1-31225 in the following applications:

  • IF/IHC
    3 publications
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MMP-12 Antibody

  • EC 3.4.24
  • hME
  • HMEEC 3.4.24.65
  • Macrophage elastase
  • macrophage metalloelastase
  • matrix metallopeptidase 12 (macrophage elastase)
  • matrix metalloproteinase 12 (macrophage elastase)
  • Matrix metalloproteinase-12
  • ME
  • MGC138506
  • MME
  • MMP12
  • MMP-12

Background

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-31225
MMP-12 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-12 Antibody (NBP1-31225)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IF/IHC, IHC, WB.


Filter By Application
IF/IHC
(3)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for MMP-12 Antibody (NBP1-31225) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-31225:
Filter by Applications
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMP-12 NBP1-31225
Enlarge 		3
reviewed by:
Ilya Ulasov
 IHC-P Human 02/22/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
Western Blot MMP-12 NBP1-31225
Enlarge 		5
reviewed by:
Kelsey Stockbridge
 WB Mouse 06/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse adipose tissue homogenate, Sample Amount: 20ug
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-12 Antibody (NBP1-31225). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody detect the catalytic domain alone of this protein?
    • The immunogen for this protein is between amino acids 213 and 307 of MMP12 (UniProt ID#P39900). This corresponds to a portion of the Hemopexin-like 1 domain on UniProt and does not cover the whole catalytic domain (ZnMc_MMP) which is between amino acids 109 and 263. Please check NCBI Gene ID: 4321 (https://www.ncbi.nlm.nih.gov/gene/?term=4321) for more information.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunohistochemistry-Paraffin MMP-12 NBP1-31225
Enlarge
 Ilya Ulasov
02/22/2017
Application: IHC-P
Species: Human
read this review
Western Blot MMP-12 NBP1-31225
Enlarge
 Kelsey Stockbridge
06/21/2011
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol MMP12