Home » MLK3 » MLK3 Antibodies » MLK3 Antibody (3D11)
Novus Biologicals products are now on bio-techne.com

MLK3 Antibody (3D11)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody (M02), clone 3D11.Lane 1: MAP3K11 transfected lysate(92.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody (M02), clone 3D11.Lane 1: MAP3K11 transfected lysate(92.7 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of monoclonal antibody to MAP3K11 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MLK3 Antibody (3D11) [H00004296-M02] - Analysis of protein-protein interactions between CDC42 and MAP3K11. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and ...read more
ELISA: MLK3 Antibody (3D11) [H00004296-M02] - Detection limit for recombinant GST tagged MAP3K11 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func, IHC
Clone
3D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals offers 100% Guarantee

MLK3 Antibody (3D11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MAP3K11 (NP_002410, 741 a.a. ~ 847 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPPGTSRSAPGTPGTPRSPPLGLISRPRPSPLRSRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFQGGPQDCRAQTKDMGAQAPWVPEAGP
Specificity
MAP3K11 - mitogen-activated protein kinase kinase kinase 11 (3D11)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAP3K11
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MLK3 Antibody (3D11)

  • EC 2.7.11
  • EC 2.7.11.25
  • MAP3K11
  • MEKK11
  • mitogen-activated protein kinase kinase kinase 11
  • Mixed lineage kinase 3
  • MLK3
  • MLK3MLK-3
  • PTK1
  • PTK1protein-tyrosine kinase PTK1
  • RHOE
  • SH3 domain-containing proline-rich kinase
  • SPRK
  • SPRKMGC17114
  • Src-homology 3 domain-containing proline-rich kinase

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western Blot JNK1 Antibody (228601) [Unconjugated]
Western Blot JNK1 Antibody (228601) [Unconjugated]
Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
MAP3K13 Antibody
NBP1-81272
Western Blot MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
JNK3 Antibody
(29E10)
NBP3-26149
Immunohistochemistry JNK3 Antibody (29E10)
Western Blot JNK3 Antibody (29E10)
Immunocytochemistry/ Immunofluorescence JNK3 Antibody (29E10)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, WB
MKK4/MEK4 Antibody
(OTI8A8)
NBP1-47839
Flow Cytometry MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
(1 Review)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(50 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
MKK7/MEK7 Antibody
(4E5) - BSA Free
NBP2-37542
ELISA MKK7/MEK7 Antibody (4E5) - BSA Free
Western Blot MKK7/MEK7 Antibody (4E5) - BSA Free
Immunocytochemistry/ Immunofluorescence MKK7/MEK7 Antibody (4E5) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
CDC42 Antibody
(JJ086-04)
NBP2-67895
Flow Cytometry CDC42 Antibody (JJ086-04)
Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)
Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
MAP3K12 Antibody
NBP2-17218
Western Blot MAP3K12 Antibody
Western Blot MAP3K12 Antibody
Immunohistochemistry-Paraffin MAP3K12 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
Glutamate Receptor
6 Antibody (4B4L9)
NBP3-16867
Western Blot Glutamate Receptor 6 Antibody (4B4L9)
Western Blot Glutamate Receptor 6 Antibody (4B4L9)
Immunohistochemistry Glutamate Receptor 6 Antibody (4B4L9)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(822 Publications)
Species: Hu
Applications: BA
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8)
Sandwich ELISA AHSA1 Antibody (1A2-A8)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
MLK3 Antibody
(3D11)
H00004296-M02
MLK3 Antibody (3D11)
Species: Hu
Applications: WB, ELISA, Func, IHC

Publications for MLK3 Antibody (H00004296-M02) (0)

There are no publications for MLK3 Antibody (H00004296-M02).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MLK3 Antibody (H00004296-M02) (0)

There are no reviews for MLK3 Antibody (H00004296-M02). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MLK3 Antibody (H00004296-M02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MLK3 Products

Array H00004296-M02

Research Areas for MLK3 Antibody (H00004296-M02)

Find related products by research area.

Blogs on MLK3

There are no specific blogs for MLK3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MLK3 Antibody (3D11) and receive a gift card or discount.


Bioinformatics

Gene Symbol MAP3K11
Entrez
Uniprot