MEK1 [p Thr386] Antibody Summary
Immunogen |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH. Accession # Q02750 |
Modification |
p Thr386 |
Specificity |
Specific for endogenous levels of the ~45 kDa MEK1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase. |
Predicted Species |
Mouse (100%), Bovine (100%), Xenopus (100%), Primate (100%), Canine (100%), Chicken (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
MAP2K1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Simple Western 1:50
- Western Blot 1:1000
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Application Notes |
NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application. See Simple Western Antibody Database for Simple Western validation: tested in HeLa + EGF; separated by size, antibody dilution of 1:50. |
Theoretical MW |
45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Publications |
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Reactivity Notes
Human reactivity reported in scientific literature (PMID: 26446942)
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Preservative |
No Preservative |
Purity |
Antigen Affinity-purified |
Alternate Names for MEK1 [p Thr386] Antibody
Background
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for MEK1 Antibody (NB500-144)(8)
Showing Publications 1 -
8 of 8.
Publications using NB500-144 |
Applications |
Species |
Chong H, Vikis HG, Guan KL. Mechanisms of regulating the Raf kinase family. Cellular Signalling 15 (2003) 463-469. [PMID: 12639709] |
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Quality Control Tests: Dot blots Western blots performed with each lot. |
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Adams, JP Sweatt, JD. Roles for the ERK MAP kinase cascade in memory, Annu. Rev Pharmacol Toxicol 42 135 - 163. 2002-01-01 [PMID: 11807168] |
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Ahn, NG, quot. The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol. Cell Biochem. 127 - 128 201 - 209. 1993-01-01 [PMID: 7935352] |
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Mansour, SJ, Resing, KA, Ci, JM, Hermann, AS, Gloor, JW, Herskind, KR, Wartmann, M, Davis, RJ, Ahn, NG, quot. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry site-directed mutagenesis. J. Biochem. (Tokyo) 116 304. 1994-01-01 [PMID: 7822248] |
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Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG. Microtubule-associated protein 2 kinases, ERK1 ERK2, undergo autophos-phorylation on both tyrosine threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88 6142-6146. 1991-01-01 [PMID: 1712480] |
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Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J. pp54 Microtubule-associated protein-2 kinase requires both tyrosine serine/threonine phosphorylation for activity. J Biol Chem 266 10043-10046. 1991-01-01 [PMID: 1645334] |
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Crews, CM, Alessrini, A, Erikson, RL. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258 478 - 480. 1992-01-01 [PMID: 1411546] |
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