Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] - Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 u/ml.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified
Alternate Names for MD-2 Antibody (1A2E3) - BSA Free
ESOP1
ESOP-1
LY96
ly-96
lymphocyte antigen 96
MD2
MD-2
myeloid differentiation protein-2
Protein MD-2
Background
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MD-2 Antibody (NBP1-75513). (Showing 1 - 1 of 1 FAQ).
Have NBP1-75513 and NBP1-76709 been tested using immunoprecipitation techniques?
Unfortunately neither of these antibodies have been validated for IP. However, we do supply alternative products; NB100-56700 for instance is another LY86 antibody which has been tested for IP. Unfortunately none of our MD2 antibodies are validated for IP. If you would like to test any of these antibodies for an untested application/species, I recommend that you take advantage of our Innovator's Reward program.
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