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MD-2 Antibody (1A2E3) - BSA Free

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Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] - Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 u/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A2E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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MD-2 Antibody (1A2E3) - BSA Free Summary

Immunogen
Antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LY96
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1 ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75513 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MD-2 Antibody (1A2E3) - BSA Free

  • ESOP1
  • ESOP-1
  • LY96
  • ly-96
  • lymphocyte antigen 96
  • MD2
  • MD-2
  • myeloid differentiation protein-2
  • Protein MD-2

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MD-2 Antibody (NBP1-75513) (0)

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Review for MD-2 Antibody (NBP1-75513) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-75513:
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WB
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 09/08/2021
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Summary

ApplicationWestern Blot
Sample TestedSpleen tissue
SpeciesMouse
Lot1A2E3

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MD-2 Antibody (NBP1-75513). (Showing 1 - 1 of 1 FAQ).

  1. Have NBP1-75513 and NBP1-76709 been tested using immunoprecipitation techniques?
    • Unfortunately neither of these antibodies have been validated for IP. However, we do supply alternative products; NB100-56700 for instance is another LY86 antibody which has been tested for IP. Unfortunately none of our MD2 antibodies are validated for IP. If you would like to test any of these antibodies for an untested application/species, I recommend that you take advantage of our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

Additional MD-2 Products

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Recent Reviews

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Verified Customer
09/08/2021
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol LY96
Uniprot