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WHSC1 Antibody (CL1063)

Images

 
Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human cerebral cortex shows moderate positivity in neuronal cell nuclei.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human testis shows nuclear immunoreactivity in a subset of cells in seminiferous tubules.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human tonsil shows nuclear positivity in the reaction centrum cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human ventricular cancer shows strong nuclear immunoreactivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL1063
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

WHSC1 Antibody (CL1063) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGD
Epitope
ALPFIPADKL
Predicted Species
Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NSD2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Control Peptide
WHSC1 Recombinant Protein Antigen (NBP2-52963PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for WHSC1 Antibody (CL1063)

  • EC 2.1.1.43
  • FLJ23286
  • IL5 promoter REII region-binding protein
  • KIAA1090
  • MGC176638
  • MMSETNSD2Multiple myeloma SET domain-containing protein
  • multiple myeloma SET domain containing protein type III
  • nuclear receptor binding SET domain protein 2
  • Nuclear SET domain-containing protein 2
  • probable histone-lysine N-methyltransferase NSD2
  • Protein trithorax-5
  • REIIBP
  • trithorax/ash1-related protein 5
  • TRX5
  • WHS
  • Wolf-Hirschhorn syndrome candidate 1 protein
  • Wolf-Hirschhorn syndrome candidate 1

Background

WHSC1 encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NSD2