LYVE-1 Antibody - BSA Free

LYVE-1 (Lymphatic Vessel Endothelial Receptor 1) is a receptor for the extracellular matrix mucopolysaccharide hyaluronan (HA) and is primarily expressed by lymphatic endothelial cells in addition to the sinusoidal endothelium of the liver and spleen (1-3). HA, also called hyaluronic acid, is a main component of the extracellular matrix and functions largely in cell adhesion, migration, and tissue remodeling (2). HA undergoes a turnover process that involves release from the tissues to the afferent lymph, degradation within the lymph nodes, and removal of fragments by the liver (2,3). LYVE-1, in addition to other lymphatic proteins including VEGFR3, Prox1, and podoplanin, is a common marker for differentiating between the blood and lymphatic systems (2,3). Furthermore, LYVE-1 is closely related to the leukocyte receptor CD44, having ~44% sequence similarity (1-3). Like CD44, the LYVE-1 protein contains an extracellular HA-binding link domain, with N- and C-terminal extensions, located at the end of a glycosylated juxtamembrane domain stalk region, followed by a transmembrane region, and a cytoplasmic tail (1-3). The key features of the HA-binding like molecule are the three disulfide bridges formed by six cysteine residues (1-3). LYVE-1 is synthesized as a protein of 322 amino acids in length with a theoretical molecular weight of 35 kDa, although due to O-glycosylation often appears in SDS-PAGE at a molecular weight ranging from ~60-70 kDa (2,4). The role of HA-binding is further elucidated by the identification of LYVE-1 as a docking receptor for dendritic cells and macrophages, binding their surface HA to control the entry and migration into lymph vessels (1).

LYVE-1 has been an important marker in studies of embryonic and tumor lymphangiogenesis, as many cancers are characterized by early metastasis to the lymph nodes (1-3, 5). One study of five different vascular tumors in infants used immunohistochemical analysis and found positive LYVE-1 expression in infantile hemangioma, tufted angioma, and kaposiform hemangioendothelioma (5). LYVE-1 along with other markers such as GLUT-1, CD31, CD34, Prox-1, and WT-1 can be used to help provide immunohistologic profiles of various tumors and, when used in conjunction with clinical and histopathologic approaches, may offer better overall diagnosis and disease treatment (5).

References

1. Jackson D. G. (2019). Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology : Journal of the International Society for Matrix Biology. https://doi.org/10.1016/j.matbio.2018.02.001

2. Jackson D. G. (2004). Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0811.x

3. Jackson D. G. (2003). The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine. https://doi.org/10.1016/s1050-1738(02)00189-5

4. Unitprot (Q9Y5Y7)

5. Johnson, E. F., Davis, D. M., Tollefson, M. M., Fritchie, K., & Gibson, L. E. (2018). Vascular Tumors in Infants: Case Report and Review of Clinical, Histopathologic, and Immunohistochemical Characteristics of Infantile Hemangioma, Pyogenic Granuloma, Noninvoluting Congenital Hemangioma, Tufted Angioma, and Kaposiform Hemangioendothelioma. The American Journal of Dermatopathology. https://doi.org/10.1097/DAD.0000000000000983

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IF, IHC, IHC-Fr, IHC-P, Immunohistochemistry.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB100-725	Applications	Species
Chaurasiya V, Pham DD, Harju J et al. Human visceral adipose tissue microvascular endothelial cell isolation and establishment of co-culture with white adipocytes to analyze cell-cell communication Experimental cell research 2023-10-16 [PMID: 37852349]
Cheon H, Gelvosa MN, Kim SA et al. Lymphatic channel sheet of polydimethylsiloxane for preventing secondary lymphedema in the rat upper limb model Bioengineering & Translational Medicine 2023-01-01 [PMID: 36684082] (Immunohistochemistry)	Immunohistochemistry
Nagahara AI, Homma J, Ryu B et al. Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels Scientific reports 2022-12-15 [PMID: 36522421] (IHC, Rat)	IHC	Rat
Griveau A, Seano G, Shelton SJ et al. A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment. Cancer Cell 2018-04-06 [PMID: 29681511] (Human)		Human
Bumb A, Regino CA, Egen JG et al. Trafficking of a dual-modality magnetic resonance and fluorescence imaging superparamagnetic iron oxide-based nanoprobe to lymph nodes Mol Imaging Biol 2011-12-01 [PMID: 21080233] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Azam SH Characterizing a Regulatory Axis of MicroRNA-200b, the RNA-Binding Protein Quaking, and Cyclin D1 in Modulating Tumor Angiogenesis and Metastasis Thesis 1905-07-11
Pique-Regi R, Romero R, Tarca AL et al. Single Cell Transcriptional Signatures of the Human Placenta in Term and Preterm Parturition bioRxiv (IF, IHC-Fr, Human)	IF, IHC-Fr	Human
Kang MJ, Lee S, Jung U Et al. Inhibition of Hepatic Stellate Cell Activation Suppresses Tumorigenicity of Hepatocellular Carcinoma in Mice The American journal of pathology 2021-08-21 [PMID: 34428424] (IHC-P, Mouse)	IHC-P	Mouse
Schafflick D, Wolbert J, Heming M Et Al. Single-cell profiling of CNS border compartment leukocytes reveals that B cells and their progenitors reside in non-diseased meninges Nature neuroscience 2021-07-12 [PMID: 34253922] (IHC-Fr)	IHC-Fr
Esposito E, Ahn BJ, Shi J et al. Brain-to-cervical lymph node signaling after stroke Nat Commun. 2019-11-22 [PMID: 31757960] (Mouse) Details: Citation used the Biotin format of this antibody.		Mouse
Cheon H, Gelvosa M, Kim S et al. Lymphatic channel sheet of polydimethylsiloxane for preventing secondary lymphedema in the rat upper limb model Bioengineering & Translational Medicine 2022-07-05
Pique-Regi R, Romero R, Tarca AL et al. Single Cell Transcriptional Signatures of the Human Placenta in Term and Preterm Parturition Elife 2019-12-13 [PMID: 31829938]
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Bryan Tinsley	WB	Human	07/01/2014	View Summary Application Western Blot Species Human
Enlarge	5 reviewed by: Corey .	IHC-P	Human	03/19/2013	View Summary Application Immunohistochemistry-Paraffin Sample Tested MDA-MB-231 breast cancer orthotopic transplantation Species Human Lot A-A

Array NB100-725

• LYVE-1 Antibodies
• LYVE-1 Antibody Pack
• LYVE-1 Lysate
• LYVE-1 Proteins
• LYVE-1 Proteins and Enzyme
• LYVE-1 ELISA