Novus Biologicals products are now on bio-techne.com

Lyn Antibody

Images

 
Western Blot: Lyn Antibody [NBP2-17189] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Lyn Antibody [NBP2-17189] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunoprecipitation: Lyn Antibody [NBP2-17189] - K562 whole cell extract A. 40 ug K562 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of LYN protein by 4 ug LYN antibody 5 % ...read more
Genetic Strategies: Western Blot: Lyn Antibody [NBP2-17189] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Lyn Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Lyn. The exact sequence is proprietary.
Predicted Species
Mouse (95%), Rat (95%), Sheep (96%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (81%), Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Lyn Antibody

  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ26625
  • Hck-2
  • JTK8
  • Lyn
  • tyrosine-protein kinase Lyn
  • v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • v-yes-1
  • Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino-terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. The human Lyn gene maps to chromosome 8q13 and encodes a 505 amino acid protein. Hematopoietic tissues predominantly express Lyn, which influences normal immunoglobulin production and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NBP1-32945
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB500-517
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF5129
Species: Hu
Applications: WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
MAB3915
Species: Hu, Mu
Applications: WB
NBP1-78295
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB7500
Species: Hu
Applications: ICC, WB
MAB6210
Species: Hu
Applications: Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
7268-CT
Species: Hu
Applications: BA
NBP1-85677
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP2-67321
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-59451
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
AF4467
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP2-17189
Species: Hu, Mu, Rt, Bv, Sh
Applications: WB, ICC/IF, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Lyn Antibody (NBP2-17189) (0)

There are no publications for Lyn Antibody (NBP2-17189).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lyn Antibody (NBP2-17189) (0)

There are no reviews for Lyn Antibody (NBP2-17189). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lyn Antibody (NBP2-17189) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Lyn Products

Research Areas for Lyn Antibody (NBP2-17189)

Find related products by research area.

Blogs on Lyn

There are no specific blogs for Lyn, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lyn Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LYN
Uniprot