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Fgr Antibody

Images

 
Western Blot: Fgr Antibody [NBP1-85677] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431
Immunocytochemistry/ Immunofluorescence: Fgr Antibody [NBP1-85677] - Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane & aggresome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human Lymph node shows moderate cytoplasmic and membranous positivity in germinal center and non-germinal center cells.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Analysis in human bone marrow and skeletal muscle tissues using NBP1-85677 antibody. Corresponding FGR RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human Bone marrow shows strong cytoplasmic and membranous positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human Cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Fgr Antibody [NBP1-85677] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Fgr Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEARTEDDLTFTKGEKFHILNNTEGDWWEARSLSSGKTGCIPSNYVAPVDSIQAEEWYFGKIG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Fgr Protein (NBP1-85677PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fgr Antibody

  • c-fgr
  • c-src-2 proto-oncogene
  • c-src2
  • EC 2.7.10
  • EC 2.7.10.2
  • Fgr
  • FLJ43153
  • Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
  • MGC75096
  • p55-c-fgr protein
  • p55c-fgr
  • p55-Fgr
  • p58c-fgr
  • Proto-oncogene c-Fgr
  • proto-oncogene tyrosine-protein kinase FGR
  • Src2
  • SRC2c-fgr protooncogene
  • tyrosine-protein kinase Fgr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FGR