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![Western Blot: Lyn Antibody [NBP2-17188] - A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract C. 30 ug Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Lyn-Antibody-Western-Blot-NBP2-17188-img0007.jpg "Western Blot: Lyn Antibody [NBP2-17188] - A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract C. 30 ug Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Lyn Antibody [NBP2-17188] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17188-img0003.jpg)
![Immunohistochemistry-Paraffin: Lyn Antibody [NBP2-17188] - Human colon carcinoma, using LYN antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunohistochemistry-Paraffin-NBP2-17188-img0002.jpg)
![Immunoprecipitation: Lyn Antibody [NBP2-17188] - IP samples: K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of LYN protein by 4 ug LYN antibody 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunoprecipitation-NBP2-17188-img0005.jpg)
![Western Blot: Lyn Antibody [NBP2-17188] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Lyn antibody (NBP2-17188) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17188_rabbit-polyclonal-lyn-antibody-910202420122030.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Lyn Antibody [NBP2-17188] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17188_rabbit-polyclonal-lyn-antibody-1610202419405711.jpg)
![Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.](http://images.novusbio.com/fullsize/Lyn-Antibody-LYN-01-Western-Blot-NB500-519-img0001.jpg "Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.")
![Western Blot: Lyn Antibody [NBP1-83072] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Lyn-Antibody-Western-Blot-NBP1-83072-img0008.jpg "Western Blot: Lyn Antibody [NBP1-83072] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: Lyn Antibody [NBP1-83072] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunohistochemistry-Paraffin-NBP1-83072-img0010.jpg)
![Western Blot: Lyn Antibody [NBP1-83072] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/Lyn-Antibody-Western-Blot-NBP1-83072-img0007.jpg)
![Immunohistochemistry-Paraffin: Lyn Antibody [NBP1-83072] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunohistochemistry-Paraffin-NBP1-83072-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lyn Antibody [NBP1-83072] - Staining in human spleen and cerebral cortex tissues using anti-LYN antibody. Corresponding LYN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunohistochemistry-Paraffin-NBP1-83072-img0011.jpg)
![Western Blot: Lyn Antibody [NBP2-17189] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Lyn-Antibody-Western-Blot-NBP2-17189-img0001.jpg "Western Blot: Lyn Antibody [NBP2-17189] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lyn Antibody [NBP2-17189] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17189-img0002.jpg)
![Immunoprecipitation: Lyn Antibody [NBP2-17189] - K562 whole cell extract A. 40 ug K562 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of LYN protein by 4 ug LYN antibody 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunoprecipitation-NBP2-17189-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Lyn Antibody [NBP2-17189] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17189_rabbit-polyclonal-lyn-antibody-1610202419414571.jpg)
![Western Blot: Lyn [p Tyr397] Antibody (HL122) [NBP3-13673] - Untreated (-) and treated (+) Raji whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lyn (phospho Tyr397) antibody (NBP3-13673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lyn-p-Tyr397-Antibody-HL122-Western-Blot-NBP3-13673-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lyn [p Tyr397] Antibody (HL122) [NBP3-13673] - Untreated (-) and treated (+) Raji whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lyn (phospho Tyr397) antibody (NBP3-13673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Lyn [p Tyr397] Antibody (HL122) [NBP3-13673] - Lyn antibody [HL122] detects Lyn protein in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Sample: Paraffin-embedded Human Matched Pair Tissue Array - Brain (Alzheimer's Disease + Normal) .Lyn stained by Lyn antibody [HL122] (NBP3-13673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13673_rabbit-lyn-p-tyr-397-mab-hl122-9102024205695.jpg)
![Immunohistochemistry-Paraffin: Lyn [p Tyr397] Antibody (HL122) [NBP3-13673] - Lyn antibody detects Lyn protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Lyn stained by Lyn antibody (NBP3-13673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13673_rabbit-lyn-p-tyr-397-mab-hl122-91020242075968.jpg)
![Western Blot: Lyn Antibody (JE44-75) [NBP3-32549] - Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (NBP3-32549) at 1/1,000 dilution. Lane 1: Ramos cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: THP-1 cell lysate (15 ug/Lane) Lane 5: Raji cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: U-937 cell lysate (15 ug/Lane) Lane 8: Mouse lung tissue lysate (30 ug/Lane) Lane 9: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32549) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32549_rabbit-lyn-mab-je44-75-278202416331240.jpg "Western Blot: Lyn Antibody (JE44-75) [NBP3-32549] - Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (NBP3-32549) at 1/1,000 dilution. Lane 1: Ramos cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: THP-1 cell lysate (15 ug/Lane) Lane 5: Raji cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: U-937 cell lysate (15 ug/Lane) Lane 8: Mouse lung tissue lysate (30 ug/Lane) Lane 9: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32549) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Lyn Antibody (JE44-75) [NBP3-32549] - Flow cytometric analysis of A431 cells labeling Lyn. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32549, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32549_rabbit-lyn-mab-je44-75-29202416453275.jpg)
![Western Blot: Lyn [p Tyr 396] Antibody (SR2011) [NBP3-21581] - Western blot analysis using NBP3-21581 in Jurkat cell lysate treated with Pervanadate.](http://images.novusbio.com/fullsize/nbp3-21581_rabbit-lyn-p-tyr-396-mab-sr2011-57202316494241.jpg "Western Blot: Lyn [p Tyr 396] Antibody (SR2011) [NBP3-21581] - Western blot analysis using NBP3-21581 in Jurkat cell lysate treated with Pervanadate.")
![Western Blot: Lyn Antibody [NB100-93453] - (0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Lyn-Antibody-Western-Blot-NB100-93453-img0002.jpg "Western Blot: Lyn Antibody [NB100-93453] - (0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Lyn [p Tyr396] Antibody (5I5G8) [NBP3-16921] - Western blot analysis of extracts of NIH/3T3 cells, using Lyn Rabbit mAb (NBP3-16921) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Lyn-p-Tyr396-Antibody-5I5G8-Western-Blot-NBP3-16921-img0001.jpg "Western Blot: Lyn [p Tyr396] Antibody (5I5G8) [NBP3-16921] - Western blot analysis of extracts of NIH/3T3 cells, using Lyn Rabbit mAb (NBP3-16921) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Lyn [p Tyr397] Antibody [NBP3-12926] - Untreated (-) and treated (+) Raji whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lyn (phospho Tyr397) antibody (NBP3-12926) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lyn-[p-Tyr397]-Antibody-Western-Blot-NBP3-12926-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lyn [p Tyr397] Antibody [NBP3-12926] - Untreated (-) and treated (+) Raji whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lyn (phospho Tyr397) antibody (NBP3-12926) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")