![Western Blot: Lumican Antibody (JE11-45) [NBP2-76847] - Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: 293T Lane 3: Human skin](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Western-Blot-NBP2-76847-img0001.jpg "Western Blot: Lumican Antibody (JE11-45) [NBP2-76847] - Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: 293T Lane 3: Human skin")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JE11-45 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human Lumican aa 172-338 / 338. (SwissProt: P51884 Human) |
| Localization | Extracellular matrix. Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LUM |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lumican Antibody (NBP2-76847)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LUM |
![Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Immunocytochemistry-Immunofluorescence-NBP2-76847-img0004.jpg "Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Lumican antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Immunohistochemistry-NBP2-76847-img0006.jpg "Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Lumican antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Lumican Antibody (JE11-45) [NBP2-76847] - Flow cytometric analysis of 293T cells with Lumican antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Flow-Cytometry-NBP2-76847-img0007.jpg "Flow Cytometry: Lumican Antibody (JE11-45) [NBP2-76847] - Flow cytometric analysis of 293T cells with Lumican antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Immunocytochemistry-Immunofluorescence-NBP2-76847-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Immunocytochemistry-Immunofluorescence-NBP2-76847-img0003.jpg "Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lumican antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lumican-Antibody-JE11-45-Immunohistochemistry-NBP2-76847-img0005.jpg "Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lumican antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")