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Human LIF ELISA Kit (Colorimetric)

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Human LIF ELISA Kit [NBP1-91186] - Data for Human LIF Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
Standard Curve Range
3.15 - 200 pg/mL
Sensitivity
0.7 pg/mL

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Human LIF ELISA Kit (Colorimetric) Summary

Description
The human LIF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human LIF
Specificity
The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human LIF positive serum. No cross-reactivity or interference was detected.
Standard Curve Range
3.15 - 200 pg/mL
Sensitivity
0.7 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.00%
Intra-Assay
CV% < 5.50%
Spike Recovery
90%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LIF

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonalantibody to human LIF
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human LIF monoclonal antibody
  5. Human LIF Standard, 400 pg/mL
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human LIF ELISA Kit (Colorimetric)

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Leukemia inhibitory factor (LIF) is a cytokine which was primarily described as a product of activated T lymphocytes and macrophages. It is a variably glycosylated 180 amino acid protein of apparent molecular weight between 35 and 67 kDa depending on its source and glycosylation. The human LIF-gene is located on chromosome 22, the gene product is highly conserved between species. The multivarious nature of LIF is illustrated by its multiple "discoveries" under a variety of synonyms. LIF exerts its pleiotropic functions in a wide variety of systems. Examples for its important and diverse physiological functions are support of growth and inhibition of differentiation of normal embryonic stem cells, stimulation of the production of acute-phase proteins by hepatocytes. A soluble form of LIF can be detected in human body fluids such as serum, whole blood, synovial fluid and urine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Applications: ELISA

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Product General Protocols

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FAQs for LIF ELISA Kit (NBP1-91186). (Showing 1 - 1 of 1 FAQ).

  1. In the product insert it is mentioned that Colored dyes for optional use with this kit are available and will be provided free of charge upon request and for the same it is advised to refer the catalog number NBP2-41404. But the status of the said pack of vials are currently showing as "discontinued". May I request you to kindly confirm whether some other dye would be offered along with this kit and if the customer needs to request for it separately.
    • For our kit NBP1-91186, we recently changed the format for this ELISA kit. The current format no longer includes the need for the colored dyes that were once included in the kit. We offer these dyes for customers that have purchased this kit in its old formulation and still require these dyes for their analysis. The dyes are no longer required for detection in kits that are purchased moving forward. If it is still intended on performing an ELISA and needs these dyes for their analysis, they can still be provided upon request.

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Research Areas for LIF ELISA Kit (NBP1-91186)

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Blogs on LIF.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol LIF