Description | A biologically active recombinant protein with a 16 residue His tag and corresponding to 204 total residues of LIF. Source:E. coli The recombinant protein migrates with an apparent molecular mass of 28-30 kDa in SDS-PAGE. |
Preparation Method |
Produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. |
Details of Functionality | The bioactivity of NovActive murine LIF was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically < 1 ng/ml, corresponding to a specific activity of > 5 x 10e7 U/mg (where 50 U is defined as the amount of mLIF required to induce differentiation of 50% of M1 cells). |
Protein/Peptide Type | Recombinant Protein |
Gene | LIF |
Endotoxin Note | Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kinetic assay. |
Dilutions |
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Application Notes | NovActiverecombinant mouse LIF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. |
Theoretical MW | 22.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | Sterile filtered 0.2 um.PBS, pH 7.2. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute with sterilized water to a final concentration of at least 0.1 mg/ml. |
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Gene Symbol | LIF |