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Lgr5/GPR49 Antibody (803420) [PE/Atto594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
803420
Clonality
Monoclonal
Host
Rat
Conjugate
PE/Atto594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8240U-100UG
Alexa Fluor 405
FAB8240V-100UG
Alexa Fluor 488
FAB8240G-100UG
Alexa Fluor 532
FAB8240X
Alexa Fluor 594
FAB8240T-100UG
Alexa Fluor 647
FAB8240R-025UG
Alexa Fluor 647
FAB8240R-100UG
Alexa Fluor 700
FAB8240N-100UG
Alexa Fluor 750
FAB8240S-100UG
Allophycocyanin
FAB8240A
Allophycocyanin/Cy7
FAB8240APCCY7
Biotin
FAB8240B
CoraFluor 1
FAB8240CL1
DyLight 350
FAB8240D
DyLight 405
FAB8240E
DyLight 488
FAB8240K
DyLight 550
FAB8240L
DyLight 594
FAB8240M
DyLight 650
FAB8240W
DyLight 680
FAB8240Y
DyLight 755
FAB8240Z
FITC
FAB8240F
HRP
FAB8240H
Janelia Fluor 525
FAB8240JF525
Janelia Fluor 549
FAB8240I
Janelia Fluor 585
FAB8240JF585
Janelia Fluor 635
FAB8240JF635
Janelia Fluor 646
FAB8240J
Janelia Fluor 669
FAB8240JF669
PE/Cy5.5
FAB8240PECY55
PE/Cy7
FAB8240PECY7
PerCP
FAB8240C
Phycoerythrin
FAB8240P-100UG
Unconjugated
MAB8240
mFluor Violet 450 SE
FAB8240MFV450
mFluor Violet 500 SE
FAB8240MFV500
mFluor Violet 610 SE
FAB8240MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB8240
Carrier Free
MAB8240
Carrier Free
MAB8240-SP
Carrier Free
FAB8240R-025UG
Carrier Free
FAB8240R-100UG
Carrier Free
FAB8240V-100UG
Carrier Free
FAB8240U-100UG
Carrier Free
FAB8240T-100UG
Carrier Free
FAB8240S-100UG
Carrier Free
FAB8240N-100UG
Carrier Free
FAB8240G-100UG
Carrier Free
FAB8240P-100UG
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Lgr5/GPR49 Antibody (803420) [PE/Atto594] Summary

Additional Information
Published as Clone RD20
Immunogen
E. coli-derived recombinant mouse Lgr5/GPR49
Lys375-Pro547
Accession # Q9Z1P4
Specificity
Detects mouse Lgr5/GPR49 in ELISA. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LGR5
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Alternate Names for Lgr5/GPR49 Antibody (803420) [PE/Atto594]

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lgr5/GPR49
Antibody (803420) [PE ...
FAB8240PEATT594
Lgr5/GPR49 Antibody (803420) [PE/Atto594]
Species: Mu
Applications: Flow

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Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
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Bioinformatics

Gene Symbol LGR5