![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-LEF1-341R-Immunohistochemistry-Paraffin-NBP3-07769-img0002.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | LEF1/341R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08435) Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary) (Uniprot: Q9UJU2) |
| Localization | Nucleus. |
| Specificity | Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LEF1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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