| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Latent TGF‑ beta bp2/LTBP‑2 Gln36-Glu1821 Accession # Q14767 |
| Specificity | Detects human Latent TGF‑ beta bp2/LTBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) LTBP-2 is observed, and less than 1% cross-reactivity with recombinant human LTBP-1 and rmLTBP-4 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | LTBP2 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
LTBP-2 (Latent TGF-beta Binding Protein 2; also C14orf141) is a 220-250 kDa secreted glycoprotein member of the LTBP family of proteins. In SDS-PAGE and under reducing conditions, LTBP-2 runs at 220-250 kDa; under nonreducing conditions, LTBP-2 runs between 205-220 kDa. Although it is described as being a TGF-beta binding protein, it does not possess the ability to bind covalently to TGF-beta . Instead, it appears to bind to heparin sulfate moieties on syndecan-4 and perlecan, plus fibrillin-1 and fibulin-5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity. In addition, LTPB-2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment. Cells known to express LTBP-2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes. Mature human LTBP-2 is 1786 amino acids (aa) in length (aa 36-1821). It possesses three heparin-binding regions (aa 94-115, 232-249, and 344-354), plus 20 EGF-like domains (aa 187-1818) that contain four interspersed TGF-beta type binding segments. Over aa 36-1821, human LTBP-2 shares 80% aa sequence identity with mouse LTBP-2.
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Andrei Turtoi |
IHC-P | Human | 01/24/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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