![Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0010.jpg "Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clone | M1-4A12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ACP2 (AAH03160, 1 a.a. ~ 423 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGKRSGWSRAALLQLLLGVNLVVMPPTQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVYDTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPYASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRLTEPVVPKDWQQECQLASGPADTEVIVALAVCGSILFLLIVLLLTVLFRMQAQPPGYRHVADGEDHA |
| Specificity | ACP2 - acid phosphatase 2, lysosomal |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACP2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunocytochemistry-Immunofluorescence-H00000053-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunohistochemistry-Paraffin-H00000053-M01-img0008.jpg "Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.")
![Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi . Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0004.jpg "Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi . Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH (36.1 kDa) used as loading control.")
![ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01] - Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-ELISA-H00000053-M01-img0009.jpg "ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01] - Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.")
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