![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0022.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL |
| Localization | Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PICALM |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin HIER pH6 retrieval is recommended |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar protein distribution across tissues to independent antibody NBP1-86659 (B).](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0018.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar protein distribution across tissues to independent antibody NBP1-86659 (B).")
![Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/PICALM-Antibody-Western-Blot-NBP1-86658-img0008.jpg "Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PICALM-Antibody-Western-Blot-NBP1-86658-img0010.jpg "Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0017.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0019.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0020.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PICALM-Antibody-Immunohistochemistry-Paraffin-NBP1-86658-img0021.jpg "Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Bioactivity TGF-beta 1 [Unconjugated]](https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg)
![Immunohistochemistry Clusterin/APOJ Antibody [Unconjugated]](https://images.novusbio.com/images2/Clusterin_AF2747_Immunohistochemistry_9945.jpg)
![Immunohistochemistry Clusterin/APOJ Antibody [Unconjugated]](https://images.novusbio.com/images2/Clusterin_AF2747_Immunohistochemistry_9946.jpg)
![Western Blot Clusterin/APOJ Antibody [Unconjugated]](https://images.novusbio.com/images/af2747_mouse-clusterin-affinity-purified-polyclonal-ab-4120241240247.jpg)
![Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Western_Blot_10680.jpg)
![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
![ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_ELISA_22233.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/DATA_SLPI_DPI00_ELISA_792.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/SLPI_DPI00_ELISA_160.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/CCL27_DCC270_ELISA_54.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/DATA_CCL27_DCC270_ELISA_615.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")