Fibulin 5/DANCE Antibody (293904) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2‑4). It is down‑regulated in the adult vasculature but is re‑expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils (6, 9‑11). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 12). Fibulin 5 is down‑regulated in many malignancies (15). When experimentally re‑expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-beta in initiating and promoting epithelial-mesenchymal transition (EMT) (13, 15). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 14).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Functional Assay, ICC, IHC, Western Blot.

Showing Publications 1 - 4 of 4.

Publications using MAB3095	Applications	Species
Tu , Kangshen, Dou , Changwei, Zheng , Xin, Li , Chao, Yang , Wei, Yao , Yingmin, Liu , Qingguan Fibulin-5 inhibits hepatocellular carcinoma cell migration and invasion by down-regulating matrix metalloproteinase-7 expression. BMC Cancer, 2014-12-12;14(0):938. 2014-12-12 [PMID: 25494879] (IHC, Western Blot, Human)	IHC, Western Blot	Human
Sideek M, Menz C, Parsi M, Gibson M LTBP-2 competes with tropoelastin for binding to fibulin-5 and heparin, and is a negative modulator of elastinogenesis. Matrix Biol, 2013-10-19;34(0):114-23. 2013-10-19 [PMID: 24148803] (Functional Assay, ICC, Human)	Functional Assay, ICC	Human
Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression. Cancer Res., 2009-07-07;69(15):6339-46. 2009-07-07 [PMID: 19584278] (IHC, Western Blot, Human, Mouse)	IHC, Western Blot	Human, Mouse
Lomas AC, Mellody KT, Freeman LJ, Bax DV, Shuttleworth CA, Kielty CM Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation. Biochem. J., 2007-08-01;405(3):417-28. 2007-08-01 [PMID: 17472576] (ICC, Mouse)	ICC	Mouse

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	ICC	Feline	05/23/2017	View Summary Application Immunocytochemistry Sample Tested Adult heart Species Feline

Array MAB3095

• Fibulin 5/DANCE Antibodies
• Fibulin 5/DANCE ELISA Kits
• Fibulin 5/DANCE Lysate
• Fibulin 5/DANCE Proteins
• Fibulin 5/DANCE Proteins and Enzyme