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LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]

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PC-3 human prostate cancer cell line was stained with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed ...read more
LAP (TGF-beta 1) was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) at 8 µg/mL for 3 hours at room ...read more
LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) at 8 µg/mL for 3 hours at room temperature. ...read more
Recombinant Human LAP (TGF‑ beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, CyTOF-ready, Flow, ICC/IF
Clone
1015022
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC77541U-100UG
Alexa Fluor 405
IC77541V-100UG
Alexa Fluor 488
IC77541G-100UG
Alexa Fluor 532
FAB77541X
Alexa Fluor 594
IC77541T-100UG
Alexa Fluor 647
IC77541R-100UG
Alexa Fluor 700
IC77541N-100UG
Alexa Fluor 750
IC77541S-100UG
Allophycocyanin
FAB77541A
Biotin
FAB77541B
DyLight 350
FAB77541D
DyLight 405
FAB77541E
DyLight 488
FAB77541K
DyLight 550
FAB77541L
DyLight 594
FAB77541M
DyLight 650
FAB77541W
DyLight 680
FAB77541Y
DyLight 755
FAB77541Z
FITC
FAB77541F
HRP
FAB77541H
Janelia Fluor 549
FAB77541I
Janelia Fluor 646
FAB77541J
PE
FAB77541P
PerCP
FAB77541C
mFluor Violet 450 SE
FAB77541MFV450
mFluor Violet 500 SE
FAB77541MFV500
mFluor Violet 610 SE
FAB77541MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB77541-SP
Carrier Free
IC77541V-100UG
Carrier Free
IC77541U-100UG
Carrier Free
IC77541T-100UG
Carrier Free
IC77541S-100UG
Carrier Free
IC77541R-100UG
Carrier Free
IC77541N-100UG
Carrier Free
IC77541G-100UG
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LAP (TGF-beta 1) Antibody (1015022) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LAP (TGF‑ beta 1)
Met1-Ser390
Accession # P01137
Specificity
Detects human LAP (TGF‑ beta 1) in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Immunocytochemistry 5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB77541
LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]
Species: Hu
Applications: ELISA, CyTOF-ready, Flow, ICC/IF

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