![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0015.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Pm, Rb, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase A/LDHA. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Porcine (90%), Chimpanzee (100%), Bovine (91%), Rabbit (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LDHA |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
| Publication using NBP2-19320 | Applications | Species |
|---|---|---|
| Silagi ES, Novais EJ, Bisetto S et al. Lactate Efflux from Intervertebral Disc Cells is Required for Maintenance of Spine Health J. Bone Miner. Res. 2019-11-06 [PMID: 31692093] (IF/IHC, IHC-P, Mouse) | IF/IHC, IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lactate Dehydrogenase A/LDHA Antibody (NBP2-19320)Find related products by research area.
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![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0017.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10% SDS-PAGE LDHA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Western-Blot-NBP2-19320-img0004.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10% SDS-PAGE LDHA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug Raw264.7 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 10% SDS-PAGE LDHA antibody dilution: 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Western-Blot-NBP2-19320-img0007.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug Raw264.7 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 10% SDS-PAGE LDHA antibody dilution: 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Western-Blot-NBP2-19320-img0012.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0010.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0002.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Lactate Dehydrogenase A/LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19320_rabbit-polyclonal-lactate-dehydrogenase-a-ldha-antibody-91020242041212.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Lactate Dehydrogenase A/LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")