![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0013.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SU39-06 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Lactate Dehydrogenase A/LDHA aa 270-310. (SwissProt: P07195 Human; SwissProt: P00338 Human; SwissProt: P16125 Mouse; SwissProt: P06151 Mouse; SwissProt: P42123 Rat; SwissProt: P04642 Rat) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LDHA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Lactate Dehydrogenase A/LDHA Antibody (NBP2-67483)Find related products by research area.
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| Gene Symbol | LDHA |
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of LOVO cells labeling Lactate Dehydrogenase with Rabbit anti-Lactate Dehydrogenase A/LDHA antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lactate Dehydrogenase antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0014.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of LOVO cells labeling Lactate Dehydrogenase with Rabbit anti-Lactate Dehydrogenase A/LDHA antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lactate Dehydrogenase antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lactate Dehydrogenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0015.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lactate Dehydrogenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Flow-Cytometry-NBP2-67483-img0001.jpg "Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0011.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase A/LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0012.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase A/LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0003.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0004.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0005.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0006.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0007.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0008.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0009.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.")
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")