![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-262202414471526.jpg "Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 5G3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human Ku80/XRCC5 [UniProt P13010] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | XRCC5 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ku80/XRCC5 Antibody (NBP3-26248)Find related products by research area.
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| Gene Symbol | XRCC5 |
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-2722024827496.jpg "Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Positive Western Blot detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: Ku80/XRCC5 Antibody at 1: 2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa Observed band size: 83 kDa](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-282202411442624.jpg "Western Blot: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Positive Western Blot detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: Ku80/XRCC5 Antibody at 1: 2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa Observed band size: 83 kDa")
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Staining of Hela Cells with Ku80/XRCC5 Antibody (5G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-28220241312241.jpg "Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Staining of Hela Cells with Ku80/XRCC5 Antibody (5G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")