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Rat KISS1 ELISA Kit (Colorimetric)

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ELISA: Rat KISS1 ELISA Kit (Colorimetric) [NBP2-76460] - Samples were spiked with high concentrations of Rat KISS1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat KISS1 ELISA Kit (Colorimetric) [NBP2-76460] - Samples were spiked with high concentrations of Rat KISS1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat KISS1 ELISA Kit (Colorimetric) [NBP2-76460] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

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Rat KISS1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.98%
Intra-Assay
CV% < 4.93%
Spike Recovery
86-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KISS1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat KISS1 ELISA Kit (Colorimetric)

  • KiSS-1 metastasis-suppressor
  • KiSS1
  • KiSS-1
  • kisspeptin
  • Kisspeptin-1
  • malignant melanoma metastasis-suppressor
  • metastasis-suppressor KiSS-1
  • Metastin
  • MGC39258

Background

KISS1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KISS1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat KISS1 ELISA
Kit (Colorimetric)
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Product PDFs

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  Rat KISS1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol KISS1