![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249413344.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1539 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary. |
| Predicted Species | Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TRIM28 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for KAP1 Antibody (NBP3-25540)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TRIM28 |
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249371524.jpg "Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249393512.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374953.jpg "Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Human tissue extract (30 ug) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374946.jpg "Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Human tissue extract (30 ug) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024939154.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024940860.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249383124.jpg "Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")