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Isoleucyl tRNA synthetase Antibody

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Immunoprecipitation:Isoleucyl tRNA synthetase AntibodyNBP3-30608] -Detection of human IARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IARS antibody NBP3-30608 (lot NBP3-30608-1) used for IP at 6 µg per reaction. IARS was also immunoprecipitated by rabbit anti-IARS antibody BL18491. For blotting immunoprecipitated IARS, NBP3-30608 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation:Isoleucyl tRNA synthetase AntibodyNBP3-30608] -Detection of human IARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Isoleucyl tRNA synthetase Antibody Summary

Immunogen
Between 1 and 50
Assay Type
[Assay Type]
Sample Volume
[Sample Volume]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IARS1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate pH 7 -8.
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for Isoleucyl tRNA synthetase Antibody

  • EC 6.1.1
  • EC 6.1.1.5
  • FLJ20736
  • IARS1
  • ILERS
  • ILRS
  • IRS
  • isoleucine tRNA ligase 1, cytoplasmic
  • Isoleucine--tRNA ligase
  • isoleucyl-tRNA synthetase
  • isoleucyl-tRNA synthetase, cytoplasmic
  • PRO0785

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IARS1