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Isoleucyl tRNA synthetase Antibody

Images

 
Western Blot: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Isoleucyl tRNA synthetase antibody detects Isoleucyl tRNA synthetase protein by western blot analysis. Various whole cell extracts (30 ug) were separated ...read more
Immunohistochemistry-Paraffin: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Rat brain. Isoleucyl tRNA synthetase antibody (NBP3-13037) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Immunoprecipitation of Isoleucyl tRNA synthetase protein from 293T whole cell extracts using 5 ug of Isoleucyl tRNA synthetase antibody ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, BvSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Isoleucyl tRNA synthetase Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of human Isoleucyl tRNA synthetase. The exact sequence is proprietary.
Predicted Species
Mouse (92%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IARS1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
Theoretical MW
144 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Isoleucyl tRNA synthetase Antibody

  • EC 6.1.1
  • EC 6.1.1.5
  • FLJ20736
  • IARS1
  • ILERS
  • ILRS
  • IRS
  • isoleucine tRNA ligase 1, cytoplasmic
  • Isoleucine--tRNA ligase
  • isoleucyl-tRNA synthetase
  • isoleucyl-tRNA synthetase, cytoplasmic
  • PRO0785

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IARS1