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Integrin alpha 1/CD49a Antibody [Unconjugated]

 (5 Publications)
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Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti- Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin a 1/CD49a at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti- Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676) ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin alpha 1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676, filled histogram) or isotype control antibody ...read more
Integrin a1/CD49a was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Sheep Anti-Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676) at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
FAB5676A
Biotin
BAF5676
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB5676A
Carrier Free
AF5676-SP
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Integrin alpha 1/CD49a Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199
Specificity
Detects human Integrin  alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin  alpha 2, rhIntegrin  alpha 11, recombinant mouse (rm) Integrin  alpha 2, and rmIntegrin  alpha 11 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ITGA1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5676 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 1/CD49a Antibody [Unconjugated]

  • CD49 antigen-like family member A
  • CD49a antigen
  • CD49a
  • Integrin alpha 1
  • integrin, alpha 1
  • ITGA1
  • Laminin and collagen receptor
  • very late activation protein 1
  • VLA-1
  • VLA1integrin alpha-1

Background

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin  alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin  alpha 1 shares 88% aa identity with mouse Integrin  alpha 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 1/CD49a Antibody (AF5676)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol ITGA1
Uniprot