Novus Biologicals products are now on bio-techne.com

ILT2/CD85j/LILRB1 Antibody (292305) [Unconjugated]

Images

 
Human peripheral blood monocytes were stained with Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20171, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
292305
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
View Available Formulations
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

ILT2/CD85j/LILRB1 Antibody (292305) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2
Gly24-His458
Accession # Q8NHL6
Specificity
Detects human LILRB1/CD85j/ILT2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ILT4, rhILT5, rhLIR6 or rhLIR8 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LILRA5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB20171 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT2/CD85j/LILRB1 Antibody (292305) [Unconjugated]

  • CD85 antigen-like family member J
  • CD85j antigen
  • CD85j
  • Ig-like transcript 2
  • ILT2
  • ILT-2
  • ILT2FLJ37515
  • Immunoglobulin-like transcript 2
  • leukocyte Ig-like receptor-1
  • Leukocyte immunoglobulin-like receptor 1
  • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 1
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB1
  • LIR1
  • LIR1LIR-1MIR-7CD85MIR7CD85 antigen
  • member 1
  • MIR7
  • Monocyte/macrophage immunoglobulin-like receptor 7

Background

The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma , an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.

  1. Allen, D. et al. (2000) Immunobiol. 202:34.
  2. Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
  3. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  4. Young, N. et al. (2001) Immunogenetics 53:270.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB2078
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
NB500-302
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
MAB1058
Species: Hu
Applications: Block, CyTOF-ready, Flow
MAB1059
Species: Hu
Applications: CyTOF-ready, Flow
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB1225
Species: Hu
Applications: Block, CyTOF-reported, Flow
NBP1-58906
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-67321
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AF2425
Species: Hu
Applications: CyTOF-ready, Flow, WB
AF3256
Species: Hu
Applications: WB
DY417
Species: Mu
Applications: ELISA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
MAB2238
Species: Hu
Applications: AgAct, CyTOF-reported, Flow
202-IL
Species: Hu
Applications: BA
MAB139
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
MAB20171
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for ILT2/CD85j/LILRB1 Antibody (MAB20171)(4)

Reviews for ILT2/CD85j/LILRB1 Antibody (MAB20171) (0)

There are no reviews for ILT2/CD85j/LILRB1 Antibody (MAB20171). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ILT2/CD85j/LILRB1 Antibody (MAB20171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILT2/CD85j/LILRB1 Antibody (292305) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol LILRA5
Uniprot