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IL-1 RAcP/IL-1 R3 Antibody - BSA Free

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Western Blot: IL-1 RAcP/IL-1 R3 Antibody [NBP1-76464] - Analysis of IL1RAP in HeLa whole cell lysate with anti-IL1RAP (C2) using NBP1-76464 at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: IL-1 RAcP/IL-1 R3 Antibody [NBP1-76464] - Staining of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: IL-1 RAcP/IL-1 R3 Antibody [NBP1-76464] - Staining of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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IL-1 RAcP/IL-1 R3 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-1RAcP. The immunogen is located within the last 50 amino acids of IL-1RAcP. Amino Acid Squence: KWKGEKSKYPQGRFWK
Specificity
This polyclonal antibody has no cross activity to other members in the IL-1 receptor family.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1RAP
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Western Blot 1 ug/ml
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IL-1 RAcP/IL-1 R3 Peptide (NBP1-76464PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody - BSA Free

  • C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
  • FLJ37788
  • IL-1 R3
  • IL-1 RAcP
  • IL-1 receptor accessory protein
  • IL-1R3
  • IL-1R-3
  • IL1RAcP
  • IL-1RAcP
  • IL1RAP
  • interleukin 1 receptor accessory protein
  • interleukin-1 receptor accessory protein beta
  • interleukin-1 receptor accessory protein

Background

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kappaB inducing kinae (NIK) and of NF-kappaB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1RAP
Uniprot