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IL-1 beta/IL-1F2 Antibody (2805) [Unconjugated]

Images

 
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. ...read more
Recombinant HumanIL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more
Effect of A1AT on whole blood IL-1 beta release. IL-1 beta production in whole blood cultures in response to LPS (1.0 μg/ml) was performed in the presence of endogenous A1AT (i.e., undiluted) or exogenously added ...read more
Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta , IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus ...read more
Effect of high glucose and IL-1 beta alone or in combination on the protein expressions of CD31, FSP1 and alpha -SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for ...read more
The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs ...read more
Effects of PKC beta on high glucose induced IL-1 beta up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKC beta inhibitors (LY317615, 0.3 μM) ...read more
Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta , IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus ...read more
The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs ...read more
The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs ...read more
The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs ...read more
The influence of blocking IL-1 beta treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1 beta . (a–f) HAECs were incubated for 48 h with anti-IL-1 beta antibodies (1000 ng/ml) in the presence of NG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, ELISA(Cap), ICC/IF
Clone
2805
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IL-1 beta/IL-1F2 Antibody (2805) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Specificity
Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta , recombinant human IL-1 alpha , rpIL-1 alpha , rrIL-1 alpha , recombinant mouse (rm) IL-1 alpha , and rmIL-1 beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL1B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.05-0.2 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human IL-1beta /IL-1F2 Biotinylated Antibody (Catalog number BAF201)
Standard: Recombinant Human IL-1beta /IL-1F2 (Catalog number 201-LB)
Reviewed Applications
Read 1 Review rated 4
using
MAB601 in the following applications:

Publications
Read Publications using
MAB601 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is covered by one or more patents, including US Patent # 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (2805) [Unconjugated]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 beta/IL-1F2 Antibody (MAB601)(63)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: Affinity Chromatography, Antibody Array Development, Array Development, ELISA (Capture), ELISA Development, ELISA Development (Capture), Electrochemiluminescent Assay, IHC, Luminex Development, Neutralization, Neutralizing, Western Blot.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
ELISA (Capture)
(1)
ELISA Development
(7)
ELISA Development (Capture)
(2)
Electrochemiluminescent Assay
(1)
IHC
(2)
Luminex Development
(4)
Neutralization
(8)
Neutralizing
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(36)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using MAB601 Applications Species
Ishitoku, M;Mokuda, S;Araki, K;Watanabe, H;Kohno, H;Sugimoto, T;Yoshida, Y;Sakaguchi, T;Masumoto, J;Hirata, S;Sugiyama, E; Tumor Necrosis Factor and Interleukin-1 beta Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation Viruses 2023-07-03 [PMID: 37515185] (Neutralization, Human) Neutralization Human
H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells Microbial pathogenesis, 2023;180(0):106120. 2023 [PMID: 37080500] (Western Blot, Human) Western Blot Human
H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells Microbial pathogenesis, 2023-04-18;180(0):106120. 2023-04-18 [PMID: 37080500] (Western Blot, Human) Western Blot Human
Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al. Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection ACS Nano 2023-02-28 [PMID: 36745820]
YY Lin, SL Chang, SC Liu, D Achudhan, YS Tsai, SW Lin, YL Chen, CC Chen, JW Chang, YC Fong, SL Hu, CH Tang Therapeutic Effects of Live Lactobacillus plantarum GKD7 in a Rat Model of Knee Osteoarthritis Nutrients, 2022-08-01;14(15):. 2022-08-01 [PMID: 35956346] (IHC, Rat) IHC Rat
SL Chang, YY Lin, SC Liu, YS Tsai, SW Lin, YL Chen, CC Chen, CY Ko, HT Chen, WC Chen, CH Tang Oral Administration of Clostridium butyricum GKB7 Ameliorates Signs of Osteoarthritis in Rats Cells, 2022-07-11;11(14):. 2022-07-11 [PMID: 35883610] (IHC, Rat) IHC Rat
ER Prather, MA Gavrilin, MD Wewers The central inflammasome adaptor protein ASC activates the inflammasome after transition from a soluble to an insoluble state The Journal of Biological Chemistry, 2022-05-11;0(0):102024. 2022-05-11 [PMID: 35568196] (ELISA (Capture), Human) ELISA (Capture) Human
JFS Pereira, C Bessa, P Matos, P Jordan Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells Cancers, 2022-03-09;14(6):. 2022-03-09 [PMID: 35326545] (Neutralization, Human) Neutralization Human
Taida Huang, Xiaomin Huang, Hui Li, Junhua Qi, Nan Wang, Yi Xu et al. Maternal Cigarette Smoke Exposure Exaggerates the Behavioral Defects and Neuronal Loss Caused by Hypoxic-Ischemic Brain Injury in Female Offspring Frontiers in Cellular Neuroscience 2022-02-18 [PMID: 35250486]
T Nakajo, T Katayoshi, N Kitajima, K Tsuji-Nait 1,25-Dihydroxyvitamin D3 attenuates IL-1beta secretion by suppressing NLRP1 inflammasome activation by upregulating the NRF2-HO-1 pathway in epidermal keratinocytes Redox Biology, 2021-12-01;48(0):102203. 2021-12-01 [PMID: 34872043] (Western Blot, Human) Western Blot Human
Show All 63 Publications.

Review for IL-1 beta/IL-1F2 Antibody (MAB601) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB601:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IL-1 beta/IL-1F2 MAB601
Enlarge
4
reviewed by:
Verified Customer
WB Human 09/21/2018
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Summary

ApplicationWestern Blot
Sample Testedhead and neck squamous cell carcinoma cells
SpeciesHuman
LotGY3715101

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FAQs for IL-1 beta/IL-1F2 Antibody (MAB601) (0)

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Secondary Antibodies

 

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Additional IL-1 beta/IL-1F2 Products

Blogs on IL-1 beta/IL-1F2.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind ...  Read full blog post.

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Verified Customer
09/21/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL1B